Jack, BrittanyMueller, David M.Fee, Ann C.Tetlow, Ashley L.Avasthi, Prachee2019-09-062019-09-062019-05-21Brittany Jack, David M. Mueller, Ann C. Fee, Ashley L. Tetlow, Prachee Avasthi, Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic, Cell Reports, Volume 27, Issue 8, 2019, Pages 2459-2467.e3, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2019.04.087.https://hdl.handle.net/1808/29541A grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml.The unicellular green alga Chlamydomonas reinhardtii is a biflagellated cell with two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Here, we probe how actin redundancy contributes to flagellar assembly. Disrupting a single actin allows complete flagellar assembly. However, when disrupting both actins using latrunculin B (LatB) treatment on the nap1 mutant background, we find that actins are necessary for flagellar growth from newly synthesized limiting flagellar proteins. Under total actin disruption, transmission electron microscopy identified an accumulation of Golgi-adjacent vesicles. We also find that there is a mislocalization of a key transition zone gating and ciliopathy protein, NPHP-4. Our experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for these actins in transport of Golgi-adjacent vesicles and flagellar incorporation of newly synthesized proteins.This article is available under the terms of the Creative Commons Attribution License (CC BY). You may copy and distribute the article, create extracts, abstracts and new works from the article, alter and revise the article, text or data mine the article and otherwise reuse the article commercially (including reuse and/or resale of the article) without permission from Elsevier. You must give appropriate credit to the original work, together with a link to the formal publication through the relevant DOI and a link to the Creative Commons user license above. You must indicate if any changes are made but not in any way that suggests the licensor endorses you or your use of the work. Permission is not required for this type of reuse.https://creativecommons.org/licenses/by/4.0/Chlamydomonas reinhardtiiciliaactintraffickingflagellar assemblyGoldi-adjacent vesiclestransition zone compositionflagellar protein synthesisArticle10.1016/j.celrep.2019.04.087https://orcid.org/0000-0002-1688-722XopenAccess