Chilton, Annemarie S.Ellis, Ashley L.Lamb, Audrey L.2015-12-292015-12-292014-10-08Chilton, Annemarie S., Ashley L. Ellis, and Audrey L. Lamb. "Structure of an Aspergillus Fumigatus Old Yellow Enzyme (EasA) Involved in Ergot Alkaloid Biosynthesis." Acta Cryst Sect F Acta Cryst Sect F Struct Biol Commun Acta Crystallogr F Struct Biol Cryst Commun Acta Crystallogr Sect F Struct Biol Commun Acta Crystallogr F Struct Biol Commun Acta Cryst F Struct Biol Commun Acta Cryst F Acta Crystallogr F Acta Crystallographica Section F Structural Biology Communications Acta Crystallogr Sect F Acta Crystallogr Sect F Struct Biol Cryst Commun 70.10 (2014): 1328-332. http://dx.doi.org/10.1107/S2053230X14018962https://hdl.handle.net/1808/19334This is the published version.The Aspergillus fumigatus old yellow enzyme (OYE) EasA reduces chanoclavine-I aldehyde to dihydrochanoclavine aldehyde and works in conjunction with festuclavine synthase at the branchpoint for ergot alkaloid pathways. The crystal structure of the FMN-loaded EasA was determined to 1.8 Å resolution. The active-site amino acids of OYE are conserved, supporting a similar mechanism for reduction of the α/β-unsaturated aldehyde. The C-terminal tail of one monomer packs into the active site of a monomer in the next asymmetric unit, which is most likely to be a crystallization artifact and not a mechanism of self-regulation.Ergot alkaloidOld yellow enzymeEasAFgaOx3Aspergillus fumigatusStructure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesisArticle10.1107/S2053230X14018962openAccess