Niyangoda, Sayuri2025-04-102025-04-102025-03-03https://hdl.handle.net/1808/36014This poster was presented at Pittconn 2025 on 03.03.2025.Zinc, the second most abundant transition metal ion in the human brain, plays a crucial role in various physiological processes, including the modulation of neurotransmission. The use of caged zinc compounds photoactivated to allow application of zinc ions into the brain with small spatial and temporal resolutions has revealed that photo-released zinc ions resulted in decreased dopamine reuptake. It was necessary to conduct research to further investigate the interaction of photo-released zinc with dopamine transporters and different dopamine receptors. For this, we studied the dopamine release and reuptake kinetics in zebrafish whole brains using fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes in the presence of different pharmacological agents such as GBR 12909 and nomifensine which are dopamine reuptake inhibitors and SB 277011A, a D3 receptor antagonist. Once the brain was harvested, it was placed in a perfusion chamber receiving a continuous flow of oxygenated artificial cerebrospinal fluid containing each of the drug to maintain viability. The brain was then perfused with a xanthone-based photocage, and at the time of data collection, light was supplied from a mercury light source, gated through a shutter under computer control and synchronized with the FSCV measurements. Electrical stimulation for dopamine release was consisted of 15 pulses at 60 Hz and a current of 350 A. The results indicated that zinc has synergistic interaction with nomifensine for dopamine transporter binding and different binding sites compared to that of GBR12909. Our results provide a foundation to explore the role of zinc in different pathway-specific modulations during neurotransmission. These findings will help to expand the current knowledge on the role Zn2+ in dopamine release and reuptake, thereby unraveling new therapeutic pathways to treat neurodegenerative diseases associated with Zn imbalance.Copyright 2025 Sayuri NiyangodadopamineZincVoltammetryZebrafishPharmacologyPresentationopenAccess