Ho, Khanh-VanHsieh, Hsien-YehRoy, AnuradhaFoote, SarahMcDonald, PeterCoggeshall, Mark V.Ito, HideyukiLei, ZhentianSumner, Lloyd W.Stewart, George C.Lin, Chung-Ho2024-06-172024-06-172023-11-01Ho KV, Hsieh HY, Roy A, Foote S, McDonald P, Coggeshall MV, Ito H, Lei Z, Sumner LW, Stewart GC, Lin CH. Quantification and characterization of biological activities of glansreginin A in black walnuts (Juglans nigra). Sci Rep. 2023 Nov 1;13(1):18860. doi: 10.1038/s41598-023-46134-8. PMID: 37914763; PMCID: PMC10620390https://hdl.handle.net/1808/35187Glansreginin A has been reported to be an indicator of the quality of walnuts (Juglans spp.). However, bioactive properties of glansreginin A have not been adequately explored. In the present study, we quantified concentrations of glansreginin A in black walnuts (Juglans nigra) using high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) and performed an array of in vitro bioassays to characterize biological activities (e.g., antibacterial, antioxidant, anticancer capacities) of this compound. Results from HPLC–MS/MS analysis indicated that glansreginin A was presented in all 12 black cultivars examined and its contents were variable among black walnut cultivars, ranged from 6.8 mg/kg (Jackson) to 47.0 mg/kg (Hay). Glansreginin A possessed moderate antibacterial activities against Gram-positive pathogens (Staphylococcus aureus and Bacillus anthracis). This compound exhibited no antioxidant activities, did not induce the activity of antioxidant response element signaling pathways, and exerted no antiproliferative effects on tumorigenic alveolar epithelial cells and non-tumorigenic lung fibroblast cells.Copyright © The Author(s) 2023 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.https://creativecommons.org/licenses/by/4.0/Mass spectrometryAssay systemsArticle10.1038/s41598-023-46134-8PMC10620390openAccess