Nayak, TaniaEdgerton-Morgan, HeatherHorio, TetsuyaXiong, YiDe Souza, Colin P.Osmani, Stephen A.Oakley, Berl R.2014-04-152014-04-152010-08-02Nayak, Tiana, Heather Edgerton-Morgan, Tetsuya Horio, Yi Xiong, Colin P. De Souza, Stephen A. Osmani, and Berl R. Oakley. 2010 “γ-Tubulin regulates the anaphase-promoting complex/cyclosome during interphase.” J Cell Biol. 190:317-330. http://dx.doi.org/10.1083/jcb.201002105https://hdl.handle.net/1808/13491A cold-sensitive γ-tubulin allele of Aspergillus nidulans, mipAD159, causes defects in mitotic and cell cycle regulation at restrictive temperatures that are apparently independent of microtubule nucleation defects. Time-lapse microscopy of fluorescently tagged mitotic regulatory proteins reveals that cyclin B, cyclin-dependent kinase 1, and the Ancdc14 phosphatase fail to accumulate in a subset of nuclei at restrictive temperatures. These nuclei are permanently removed from the cell cycle, whereas other nuclei, in the same multinucleate cell, cycle normally, accumulating and degrading these proteins. After each mitosis, additional daughter nuclei fail to accumulate these proteins, resulting in an increase in noncycling nuclei over time and consequent inhibition of growth. Extensive analyses reveal that these noncycling nuclei result from a nuclear autonomous, microtubule-independent failure of inactivation of the anaphase-promoting complex/cyclosome. Thus, γ-tubulin functions to regulate this key mitotic and cell cycle regulatory complex.This article is distributed under the terms of an Attribution Noncommercial Share Alike No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution Noncommercial Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).http://creativecommons.org/licenses/by-nc-sa/3.0/Article10.1083/jcb.201002105openAccess