Now showing items 1-20 of 20658

    • Spatially and temporally varying selection on intra-population QTL for a life history tradeoff in Mimulus guttatus 

      Mojica, Julius P.; Lee, Young Wha; Willis, John H.; Kelly, John K. (Wiley, 2012-08)
      Why do populations remain genetically variable despite strong continuous natural selection? Mutation reconstitutes variation eliminated by selection and genetic drift, but theoretical and experimental studies each suggest ...
    • Swe1Wee1-dependent tyrosine phosphorylation of Hsp90 regulates distinct facets of chaperone function 

      Mollapour, Mehdi; Tsutsumi, Shinji; Donnelly, Alison C.; Beebe, Kristin; Tokita, Mari J.; Lee, Min-Jung; Lee, Sunmin; Morra, Giulia; Bourboulia, Dimitra; Scroggins, Bradley T.; Colombo, Giorgio; Blagg, Brian S. J.; Panaretou, Barry; Stetler-Stevenson, William G.; Trepel, Jane B.; Piper, Peter W.; Prodromou, Chrisostomos; Pearl, Laurence H.; Neckers, Len (Elsevier, 2010-02-12)
      Swe1 (Saccharomyces WEE1), the only “true” tyrosine kinase in budding yeast, is an Hsp90 client protein. Here we show that Swe1Wee1 phosphorylates a conserved tyrosine residue (Y24 in yeast Hsp90 and Y38 in human Hsp90α) ...
    • Discrimination of soluble and aggregation-prone proteins based on sequence information 

      Fang, Yaping; Fang, Jianwen (Royal Society of Chemistry, 2013-04-05)
      Understanding the factors governing protein solubility is a key to grasp the mechanisms of protein solubility and may provide insight into protein aggregation and misfolding related diseases such as Alzheimer’s disease. ...
    • A de novo protein binding pair by computational design and directed evolution 

      Karanicolas, John; Corn, Jacob E.; Chen, Irwin; Joachimiak, Lukasz A.; Dym, Orly; Peck, Sun H.; Albeck, Shira; Unger, Tamar; Hu, Wenxin; Liu, Gaohua; Delbecq, Scott; Montelione, Gaetano; Spiegel, Clint; Liu, David R.; Baker, David (Elsevier, 2011-04-22)
      The de novo design of protein-protein interfaces is a stringent test of our understanding of the principles underlying protein-protein interactions and would enable new approaches to biological and medical challenges. Here ...
    • Production, purification, and characterization of recombinant hFSH glycoforms for functional studies 

      Butnev, Viktor Y.; Butnev, Vladimir Y.; May, Jeffrey V.; Shuai, Bin; Tran, Patrick; White, William K.; Brown, Alan; Hall, Aaron Smalter; Harvey, David J.; Bousfield, George R. (Elsevier, 2015-04-15)
      Previously, our laboratory demonstrated the existence of a β-subunit glycosylation-deficient human FSH glycoform, hFSH21. A third variant, hFSH18, has recently been detected in FSH glycoforms isolated from purified pituitary ...
    • Drug Repurposing for Gastrointestinal Stromal Tumor 

      Pessetto, Ziyan Y.; Weir, Scott J.; Sethi, Geetika; Broward, Melinda A.; Godwin, Andrew K. (American Association for Cancer Research, 2013-07)
      Despite significant treatment advances over the past decade, metastatic gastrointestinal stromal tumor (GIST) remains largely incurable. Rare diseases, such as GIST, individually affect small groups of patients but ...
    • Molecular genetic analysis of the orsellinic acid/F9775 gene cluster of Aspergillus nidulans†,‡ 

      Sanchez, James F.; Chiang, Yi-Ming; Szewczyk, Edyta; Davidson, Ashley D.; Ahuja, Manmeet; Oakley, C. Elizabeth; Bok, Jin Woo; Keller, Nancy; Oakley, Berl R.; Wang, Clay C. C. (Royal Society of Chemistry, 2010-03)
      F-9775A and F-9775B are cathepsin K inhibitors that arise from a chromatin remodelling deletant strain of Aspergillus nidulans. A polyketide synthase gene has been determined to be responsible for their formation and for ...
    • Nutrient excess and altered mitochondrial proteome and function contribute to neurodegeneration in diabetes 

      Chowdhury, Subir K. Roy; Dobrowsky, Rick T.; Fennyhough, Paul (Elsevier, 2011-11-01)
      Diabetic neuropathy is a major complication of diabetes that results in the progressive deterioration of the sensory nervous system. Mitochondrial dysfunction has been proposed to play an important role in the pathogenesis ...
    • Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells 

      Mochin, Maria T.; Underwood, Karen F.; Cooper, Brandon; McLenithan, John C.; Pierce, Adam D.; Nalvarte, Cesar; Arbiser, Jack; Karlsson, Anna I.; Moise, Alexander R.; Passaniti, Antonino (Elsevier, 2015-01)
      Angiogenesis is regulated by hyperglycemic conditions, which can induce cellular stress responses, reactive oxygen species (ROS), and anti-oxidant defenses that modulate intracellular signaling to prevent oxidative damage. ...
    • Fiber Laser Based Two-Photon FRET Measurement of Calmodulin and mCherry-E0GFP Proteins 

      Adany, Peter; Johnson, Carey K.; Hui, Rongqing (Wiley, 2012-06)
      The speed and accuracy of Förster Resonance Energy Transfer (FRET) measurements can be improved by rapidly alternating excitation wavelengths between the donor and acceptor fluorophore. We demonstrate FRET efficiency ...
    • Ensemble Methods for Monitoring Enzyme Translocation along Single Stranded Nucleic Acids 

      Tomko, Eric J.; Fischer, Christopher J.; Lohman, Timothy M. (Elsevier, 2010-07)
      We review transient kinetic methods developed to study the mechanism of translocation of nucleic acid motor proteins. One useful stopped-flow fluorescence method monitors arrival of the translocase at the end of a fluorescently ...
    • The recruitment of knowledge regarding plurality and compound formation during language comprehension 

      Fiorentino, Robert; Bost, Jamie; Abel, Alyson D.; Zuccarelli, Jordan (John Benjamins Publishing, 2012-01-01)
      Compound formation has been a major focus of research and debate in mental lexicon research. In particular, it has been widely observed that compounds with a regular plural non-head are dispreferred, and a long line of ...
    • Indirect and direct relations between aerobic fitness, physical activity, and academic achievement in elementary school students 

      Lambourne, Kate; Hansen, David M.; Szabo, Amanda N.; Lee, Jaehoon; Herrmann, Stephen D.; Donnelly, Joseph E. (Elsevier, 2013-10)
      BACKGROUND: There is evidence to suggest that increasing physical activity (PA) improves academic achievement (AA) in children and that aerobic fitness is associated with both cognitive function and AA. However, it is not ...
    • Triazole Containing Novobiocin and Biphenyl Amides as Hsp90 C-Terminal Inhibitors 

      Zhao, Jinbo; Zhao, Huiping; Hall, Jessica A.; Brown, Douglas; Brandes, Eileen; Joseph, Bazzill; Grogan, Patrick T.; Subramanian, Chitra; Vielhauer, George; Cohen, Mark S.; Blagg, Brian S. J. (Royal Society of Chemistry, 2017-09-01)
      Hsp90 C-terminal inhibitors are advantageous for the development of new cancer chemotherapeutics due to their ability to segregate client protein degradation from induction of the prosurvival heat shock response, which is ...
    • Potential Drug Abuse Therapeutics Derived from the Hallucinogenic Natural Product Salvinorin A 

      Prevatt-Smith, Katherine M.; Lovell, Kimberly M.; Simpson, Denise S.; Day, Victor W.; Douglas, Justin T.; Bosch, Peter; Dersch, Christina M.; Rothman, Richard B.; Kivell, Bronwyn; Prisinzano, Thomas E. (Royal Society of Chemistry, 2011-12)
      Previous structure-activity relationship studies of salvinorin A have shown that modification of the acetate functionality off the C-2 position to a methoxy methyl or methoxy ethyl ether moiety leads to increased potency ...
    • Identification and molecular genetic analysis of the cichorine gene cluster in Aspergillus nidulans 

      Sanchez, James F.; Entwistle, Ruth; Corcoran, David; Oakley, Berl R.; Wang, Clay C. C. (Royal Society of Chemistry, 2012-08-01)
      We recently demonstrated that the phytotoxin cichorine is produced by Aspergillus nidulans. Through a set of targeted deletions, we have found a cluster of seven genes that are required for its biosynthesis. Two of the ...
    • Response 

      Melanson, Edward L.; Keadle, Sarah Kozey; Donnelly, Joseph E.; Braun, Barry; King, Neil A. (Lippincott, Williams & Wilkins, 2014-02)
    • Resistance to exercise-induced weight loss: compensatory behavioral adaptations 

      Melanson, Edward L.; Keadle, Sarah Kozey; Donnelly, Joseph E.; Braun, Barry; King, Neil A. (Lippincott, Williams & Wilkins, 2013-08)
      In many interventions that are based on an exercise program intended to induce weight loss, the mean weight loss observed is modest and sometimes far less than the individual expected. The individual responses are also ...
    • Minimal resistance training improves daily energy expenditure and fat oxidation 

      Kirk, Erik P.; Donnelly, Joseph E.; Smith, Bryan K.; Honas, Jeff; LeCheminant, James D.; Bailey, Bruce W.; Jacobsen, Dennis J.; Washburn, Richard A. (Lippincott, Williams & Wilkins, 2009-05)
      ABSTRACT: Long-term resistance training (RT) may result in a chronic increase in 24-hour energy expenditure (EE) and fat oxidation to a level sufficient to assist in maintaining energy balance and prevent weight gain. ...
    • Linear/Nonlinear Relations of Activity and Fitness with Children’s Academic Achievement 

      Hansen, David M.; Herrmann, Stephen D.; Lambourne, Kate; Lee, Jaehoon; Donnelly, Joseph E. (Lippincott, Williams & Wilkins, 2014-12)
      ABSTRACT: A growing research base suggests the benefits of physical activity (PA) and aerobic fitness for children extend beyond overall health/well-being to include academic achievement (AA). The majority of research ...