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dc.contributor.authorZhang, Shaobing O
dc.contributor.authorTrimble, Rhonda
dc.contributor.authorGuo, Fengli
dc.contributor.authorMak, Ho Yi
dc.date.accessioned2011-01-07T19:19:30Z
dc.date.available2011-01-07T19:19:30Z
dc.date.issued2010-12-08en_US
dc.identifier.urihttp://hdl.handle.net/2271/917en_US
dc.description.abstractAbstract Background Lipid droplets are a class of eukaryotic cell organelles for storage of neutral fat such as triacylglycerol (TAG) and cholesterol ester (CE). We and others have recently reported that lysosome-related organelles (LROs) are not fat storage structures in the nematode C. elegans. We also reported the formation of enlarged lipid droplets in a class of peroxisomal fatty acid β-oxidation mutants. In the present study, we seek to provide further evidence on the organelle nature and biophysical properties of fat storage structures in wild-type and mutant C. elegans. Results In this study, we provide biochemical, histological and ultrastructural evidence of lipid droplets in wild-type and mutant C. elegans that lack lysosome related organelles (LROs). The formation of lipid droplets and the targeting of BODIPY fatty acid analogs to lipid droplets in live animals are not dependent on lysosomal trafficking or peroxisome dysfunction. However, the targeting of Nile Red to lipid droplets in live animals occurs only in mutants with defective peroxisomes. Nile Red labelled-lipid droplets are characterized by a fluorescence emission spectrum distinct from that of Nile Red labelled-LROs. Moreover, we show that the recently developed post-fix Nile Red staining method labels lipid droplets exclusively. Conclusions Our results demonstrate lipid droplets as ubiquitous fat storage organelles and provide a unified explanation for previous studies on fat labelling methods in C. elegans. These results have important applications to the studies of fat storage and lipid droplet regulation in the powerful genetic system, C. elegans.en_US
dc.titleLipid droplets as ubiquitous fat storage organelles in C. elegans
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-2121-11-96en_US
dc.date.updated2010-12-20T11:03:10Z
dc.description.versionPeer Reviewed
dc.rights.holderZhang et al.; licensee BioMed Central Ltd.
dc.rights.accessrightsopenAccessen_US


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