dc.contributor.author | Chappa, Arvind K. | |
dc.contributor.author | Cooper, Joshua D. | |
dc.contributor.author | Audus, Kenneth L. | |
dc.contributor.author | Lunte, Susan M. | |
dc.date.accessioned | 2011-05-20T21:39:11Z | |
dc.date.available | 2011-05-20T21:39:11Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Chappa, A.K., Cooper, J.D., Audus, K.L., and Lunte, S.M. (2007) Investigation of the metabolism of Substance-P at the blood-brain barrier using LC-MS/MS. J. Pharm. Biomed. Anal.43, 1409-1415. PMID: 17118606 http://dx.doi.org/10.1016/j.jpba.2006.10.005 | |
dc.identifier.uri | http://hdl.handle.net/1808/7485 | |
dc.description | Please note that this is an author-produced PDF of an article accepted for publication following peer review. The publisher version is available on its site. | |
dc.description.abstract | Substance P (SP) has been associated with pain, depression as well as neurodegenerative diseases. Many of these diverse actions of SP can potentially be attributed to SP metabolites generated at the blood-brain barrier (BBB). In these studies, the metabolism of SP was investigated using an in vitro model of the BBB and LC-MS/MS. Substance P metabolism was found to be non-saturable in the concentration range of 100 nM to 10 μM, with approximately 70% of the peptide remaining intact after 5 hrs. The major metabolites of SP were identified by MS to be 3-11 and 5-11. Two previously unreported metabolites, 5-11 and 6-11 were also found in our studies. Several additional minor SP metabolites including 1-9 and 2-11 were also identified. A profile of the SP metabolites generated by the BBB overtime was obtained. The results from the present study provide us a better understanding of the role of blood-brain barrier in the pharmacology of SP. | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | |
dc.subject | Substance p | |
dc.subject | Blood-brain barrier | |
dc.subject | Lc-ms/ms | |
dc.title | Investigation of the metabolism of Substance-P at the blood-brain barrier using LC-MS/MS | |
dc.type | Article | |
kusw.kuauthor | Audus, Kenneth L. | |
kusw.kudepartment | School of Pharmacy | |
kusw.oastatus | fullparticipation | |
dc.identifier.doi | 10.1016/j.jpba.2006.10.005 | |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |