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dc.contributor.authorChappa, Arvind K.
dc.contributor.authorCooper, Joshua D.
dc.contributor.authorAudus, Kenneth L.
dc.contributor.authorLunte, Susan M.
dc.date.accessioned2011-05-20T21:39:11Z
dc.date.available2011-05-20T21:39:11Z
dc.date.issued2007
dc.identifier.citationChappa, A.K., Cooper, J.D., Audus, K.L., and Lunte, S.M. (2007) Investigation of the metabolism of Substance-P at the blood-brain barrier using LC-MS/MS. J. Pharm. Biomed. Anal.43, 1409-1415. PMID: 17118606 http://dx.doi.org/10.1016/j.jpba.2006.10.005
dc.identifier.urihttp://hdl.handle.net/1808/7485
dc.descriptionPlease note that this is an author-produced PDF of an article accepted for publication following peer review. The publisher version is available on its site.
dc.description.abstractSubstance P (SP) has been associated with pain, depression as well as neurodegenerative diseases. Many of these diverse actions of SP can potentially be attributed to SP metabolites generated at the blood-brain barrier (BBB). In these studies, the metabolism of SP was investigated using an in vitro model of the BBB and LC-MS/MS. Substance P metabolism was found to be non-saturable in the concentration range of 100 nM to 10 μM, with approximately 70% of the peptide remaining intact after 5 hrs. The major metabolites of SP were identified by MS to be 3-11 and 5-11. Two previously unreported metabolites, 5-11 and 6-11 were also found in our studies. Several additional minor SP metabolites including 1-9 and 2-11 were also identified. A profile of the SP metabolites generated by the BBB overtime was obtained. The results from the present study provide us a better understanding of the role of blood-brain barrier in the pharmacology of SP.
dc.language.isoen_US
dc.publisherElsevier
dc.subjectSubstance p
dc.subjectBlood-brain barrier
dc.subjectLc-ms/ms
dc.titleInvestigation of the metabolism of Substance-P at the blood-brain barrier using LC-MS/MS
dc.typeArticle
kusw.kuauthorAudus, Kenneth L.
kusw.kudepartmentSchool of Pharmacy
kusw.oastatusfullparticipation
dc.identifier.doi10.1016/j.jpba.2006.10.005
kusw.oaversionScholarly/refereed, author accepted manuscript
kusw.oapolicyThis item meets KU Open Access policy criteria.
dc.rights.accessrightsopenAccess


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