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    NUCLEAR FUNCTIONS OF ADENOMATOUS POLYPOSIS COLI: REGULATION OF THE G2-M CELL CYCLE TRANSITION & INTERMEDIATE FILAMENT INTERACTION

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    Wang_ku_0099D_10176_DATA_1.pdf (9.177Mb)
    Issue Date
    2009-01-22
    Author
    Wang, Yang
    Publisher
    University of Kansas
    Format
    180 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Molecular Biosciences
    Rights
    This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author.
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    Abstract
    Mutations in Adenomatous Polyposis Coli (APC) initiate most colorectal cancers. APC is implicated in regulating cell cycle and the cytoskeleton. I identified topoisomerase IIalpha (topo IIalpha), a regulator of G2 decatenation checkpoint, in complex with APC. Topo IIalpha interacts with two separate domains in the middle of APC, M2- and M3-APC. Expression of either domain resulted in G2 cell cycle arrest, likely a response to inhibition of topo IIalpha activity. However, the G2 arrest only occurred in cells containing normal levels of topo IIalpha. In a related study, I characterized a new APC antibody we generated and purified. Using this novel reagent to immunoprecipitate APC protein, I identified 42 potential APC binding proteins. Among these, intermediate filament protein lamin B1 was verified to interact with APC in both cultured cells and human tissue. Together, I provide evidence supporting a role for APC in G2-M cell cycle transition and in cytoskeletal regulation.
    URI
    http://hdl.handle.net/1808/5459
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    • Dissertations [3958]
    • Molecular Biosciences Dissertations and Theses [182]

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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