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dc.contributor.authorMacdonald, Stuart J.
dc.contributor.authorHighfill, Chad A.
dc.date.accessioned2020-06-17T20:01:17Z
dc.date.available2020-06-17T20:01:17Z
dc.date.issued2020-03-30
dc.identifier.citationMacdonald, S. J., & Highfill, C. A. (2020). A naturally-occurring 22-bp coding deletion in Ugt86Dd reduces nicotine resistance in Drosophila melanogaster. BMC research notes, 13(1), 188. https://doi.org/10.1186/s13104-020-05035-zen_US
dc.identifier.urihttp://hdl.handle.net/1808/30538
dc.descriptionThis work is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.description.abstractObjective Segregating genetic variants contribute to the response to toxic, xenobiotic compounds, and identifying these causative sites can help describe the mechanisms underlying metabolism of toxic compounds. In previous work we implicated the detoxification gene Ugt86Dd in the genetic control of larval nicotine resistance in Drosophila melanogaster. Furthermore, we suggested that a naturally-occurring 22-bp deletion that leads to a stop codon in exon 2 of the gene markedly reduces resistance. Here we use homology directed CRISPR/Cas9 gene editing to specifically test this hypothesis.

Results We edited chromosome three from an inbred strain named A4 which carries the insertion allele at Ugt86Dd, successfully generated four alleles carrying the 22-bp Ugt86Dd deletion, and substituted edited chromosomes back into the A4 background. The original A4 strain, and an un-edited control strain in the same A4 background, show no significant difference in egg-to-adult or larva-to-adult viability on either control media or nicotine-supplemented media, and only slightly delayed development in nicotine media. However, strains carrying the 22-bp deletion showed reduced viability in nicotine conditions, and significantly longer development. Our data strongly suggest that the naturally-occurring 22-bp insertion/deletion event in Ugt86Dd directly impacts variation in nicotine resistance in D. melanogaster.
en_US
dc.publisherBMCen_US
dc.rights© The Author(s) 2020.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectUgt86Dden_US
dc.subjectUgt35C1en_US
dc.subjectDrosophilaen_US
dc.subjectNicotineen_US
dc.subjectCRISPRen_US
dc.subjectQTLen_US
dc.subjectDSPRen_US
dc.titleA naturally-occurring 22-bp coding deletion in Ugt86Dd reduces nicotine resistance in Drosophila melanogasteren_US
dc.typeArticleen_US
kusw.kuauthorMacdonald, Stuart J.
kusw.kuauthorHighfill, Chad A.
kusw.kudepartmentMolecular Biosciencesen_US
kusw.kudepartmentCenter for Computational Biologyen_US
dc.identifier.doi10.1186/s13104-020-05035-zen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-9421-002Xen_US
kusw.oaversionScholarly/refereed, publisher versionen_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC7106894en_US
dc.rights.accessrightsopenAccessen_US


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Except where otherwise noted, this item's license is described as: © The Author(s) 2020.