KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Mitochondrial Haplogrouping and Short Tandem Repeat Analyses in Anthropological Research using Next-Generation Sequencing Technologies

    Thumbnail
    View/Open
    Wood_ku_0099D_16067_DATA_1.pdf (3.136Mb)
    Issue Date
    2018-08-31
    Author
    Wood, Melody Ratliff
    Publisher
    University of Kansas
    Format
    170 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Anthropology
    Rights
    Copyright held by the author.
    Metadata
    Show full item record
    Abstract
    The field of anthropological genetics aims to reveal, characterize, and understand the biological diversity of modern and ancient human populations. This goal is achieved by analyzing different regions of the autosomes, sex chromosomes, and mitochondrial genome. The last decade has introduced a new wave of technologies known as next-generation sequencing (NGS) technologies with high throughput and increased data output. NGS has been employed in the medical and forensic fields but is slow to take hold in anthropological genetics. This work demonstrates the utility of NGS to answer anthropological questions and genetically characterize populations. The accuracy of mitochondrial haplogrouping using smaller ranges of the mitogenome was assessed. When using less than the full mitogenome, haplogrouping was accurate for 95% of samples. Using only the control region, 50% of samples were precisely haplogrouped and 82% of Native American haplogroups were distinguishable from Asian haplogroups. Examining autosomal and Y-chromosome STRs, nine loci exhibited increased sequence-based allelic diversity. Five loci (D2S441, D7S820, vWA, DYS392, DYS635) demonstrated statistical differences in the frequency distributions of length-based and sequence-based alleles for Native American and Asian samples; two of these loci (vWA and DYS635) demonstrated higher significance levels when using sequence-based alleles. One locus (D2S1338) demonstrated statistical differences in the sequence-based alleles alone. This indicates the D2S1338, vWA, and DYS635 loci are populationally informative using sequence-based alleles obtained by NGS. These are some of the fundamental areas in which anthropological genetics can advance using next-generation sequencing technologies.
    URI
    http://hdl.handle.net/1808/28016
    Collections
    • Dissertations [3958]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps