Non-toxic engineered carbon nanodiamond concentrations induce oxidative/nitrosative stress, imbalance of energy metabolism, and mitochondrial dysfunction in microglial and alveolar basal epithelial cells
dc.contributor.author | Fresta, Claudia G. | |
dc.contributor.author | Chakraborty, Aishik | |
dc.contributor.author | Wijesinghe, Manjula B. | |
dc.contributor.author | Amorini, Angela M. | |
dc.contributor.author | Lazzarino, Giacomo | |
dc.contributor.author | Tavazzi, Barbara | |
dc.contributor.author | Lunte, Susan M. | |
dc.contributor.author | Caraci, Filippo | |
dc.contributor.author | Dhar, Prajnaparamita | |
dc.contributor.author | Caruso, Giuseppe | |
dc.date.accessioned | 2018-06-13T18:45:58Z | |
dc.date.available | 2018-06-13T18:45:58Z | |
dc.date.issued | 2018-02-14 | |
dc.identifier.citation | Fresta, C. G., Chakraborty, A., Wijesinghe, M. B., Amorini, A. M., Lazzarino, G., Lazzarino, G., … Caruso, G. (2018). Non-toxic engineered carbon nanodiamond concentrations induce oxidative/nitrosative stress, imbalance of energy metabolism, and mitochondrial dysfunction in microglial and alveolar basal epithelial cells. Cell Death & Disease, 9(2), 245. http://doi.org/10.1038/s41419-018-0280-z | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/26508 | |
dc.description.abstract | Engineered nanoparticles are finding a wide spectrum of biomedical applications, including drug delivery and capacity to trigger cytotoxic phenomena, potentially useful against tumor cells. The full understanding of their biosafety and interactions with cell processes is mandatory. Using microglial (BV-2) and alveolar basal epithelial (A549) cells, in this study we determined the effects of engineered carbon nanodiamonds (ECNs) on cell viability, nitric oxide (NO) and reactive oxygen species (ROS) production, as well as on energy metabolism. Particularly, we initially measured decrease in cell viability as a function of increasing ECNs doses, finding similar cytotoxic ECN effects in the two cell lines. Subsequently, using apparently non-cytotoxic ECN concentrations (2 µg/mL causing decrease in cell number < 5%) we determined NO and ROS production, and measured the concentrations of compounds related to energy metabolism, mitochondrial functions, oxido-reductive reactions, and antioxidant defences. We found that in both cell lines non-cytotoxic ECN concentrations increased NO and ROS production with sustained oxidative/nitrosative stress, and caused energy metabolism imbalance (decrease in high energy phosphates and nicotinic coenzymes) and mitochondrial malfunctioning (decrease in ATP/ADP ratio).These results underline the importance to deeply investigate the molecular and biochemical changes occurring upon the interaction of ECNs (and nanoparticles in general) with living cells, even at apparently non-toxic concentration. Since the use of ECNs in biomedical field is attracting increasing attention the complete evaluation of their biosafety, toxicity and/or possible side effects both in vitro and in vivo is mandatory before these highly promising tools might find the correct application. | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | Copyright © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.title | Non-toxic engineered carbon nanodiamond concentrations induce oxidative/nitrosative stress, imbalance of energy metabolism, and mitochondrial dysfunction in microglial and alveolar basal epithelial cells | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Fresta, Claudia G. | |
kusw.kuauthor | Chakraborty, Aishik | |
kusw.kuauthor | Wijesinghe, Manjula B. | |
kusw.kuauthor | Lunte, Susan M. | |
kusw.kuauthor | Dhar, Prajnaparamita | |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1038/s41419-018-0280-z | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1571-5327 | |
kusw.oaversion | Scholarly/refereed, publisher version | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: Copyright © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.