KUKU

KU ScholarWorks

  • myKU
  • Email
  • Enroll & Pay
  • KU Directory
    • Login
    View Item 
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    •   KU ScholarWorks
    • Dissertations and Theses
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Epigenetics of Crohn’s Disease, Ulcerative Colitis, and Phenotypically Normal Individuals

    Thumbnail
    View/Open
    Phillips_ku_0099D_15274_DATA_1.pdf (5.176Mb)
    Issue Date
    2017-05-31
    Author
    Phillips, Delisa L.
    Publisher
    University of Kansas
    Format
    210 pages
    Type
    Dissertation
    Degree Level
    Ph.D.
    Discipline
    Anthropology
    Rights
    Copyright held by the author.
    Metadata
    Show full item record
    Abstract
    Background It is difficult to distinguish between Crohn’s disease and ulcerative colitis, as they share many symptoms and many susceptibility genetic loci. Due to the similarities in phenotype between Crohn’s disease and ulcerative colitis, many patients are misdiagnosed for years. For this project, I collected genetic material, medical histories, and environmental variables from individuals with Crohn’s disease, individuals with ulcerative colitis, and individuals that are phenotypically normal. The purpose of this project is to determine the differential epigenetic methylation of genes in individuals with Crohn’s disease and ulcerative colitis compared to phenotypically normal controls. Materials and Methods Participants were recruited for this project at the Kansas Medical Center. Twenty of these individuals had Crohn’s disease, ten had ulcerative colitis, and thirty-one were phenotypically normal. Buccal swabs and information relevant to this project (via a questionnaire) was collected from the individuals. DNA was extracted then bisulfite-converted so that the unmethylated cytosines would be converted to uracils. Real-time PCR was performed on the promoter regions of three genes both prior to and post-bisulfite conversion, as well as on the regions of the genes containing known associated SNPs. The three genes of interest in this project are the NOD2, ATG16L1, and PTPN2 genes. Each of these genes has been found to be associated with Crohn’s disease. These three genes work in concert by identifying pathogenic bacteria, forming a response, and ultimately clearing out the bacteria. Results The statistical tests used in this project showed a few significant differences between Crohn’s disease, ulcerative colitis, and phenotypically normal samples from the questionnaire. From the bisulfite-converted samples, however, there were no obvious differences between the three groups.
    URI
    http://hdl.handle.net/1808/26347
    Collections
    • Dissertations [3958]

    Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.


    We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.


    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    Browse

    All of KU ScholarWorksCommunities & CollectionsThis Collection

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

    The University of Kansas
      Contact KU ScholarWorks
    Lawrence, KS | Maps
     
    • Academics
    • Admission
    • Alumni
    • Athletics
    • Campuses
    • Giving
    • Jobs

    The University of Kansas prohibits discrimination on the basis of race, color, ethnicity, religion, sex, national origin, age, ancestry, disability, status as a veteran, sexual orientation, marital status, parental status, gender identity, gender expression and genetic information in the University’s programs and activities. The following person has been designated to handle inquiries regarding the non-discrimination policies: Director of the Office of Institutional Opportunity and Access, IOA@ku.edu, 1246 W. Campus Road, Room 153A, Lawrence, KS, 66045, (785)864-6414, 711 TTY.

     Contact KU
    Lawrence, KS | Maps