Optical-flow based non-invasive analysis of cardiomyocyte contractility
| dc.contributor.author | Czirok, Andras | |
| dc.contributor.author | Isai, Dona Greta | |
| dc.contributor.author | Kosa, Edina | |
| dc.contributor.author | Rajasingh, Sheeja | |
| dc.contributor.author | Kinsey, William | |
| dc.contributor.author | Neufeld, Zoltan | |
| dc.contributor.author | Johnson, Rajasingh | |
| dc.date.accessioned | 2018-02-21T17:03:26Z | |
| dc.date.available | 2018-02-21T17:03:26Z | |
| dc.date.issued | 2017-09-04 | |
| dc.identifier.citation | Czirok, A., Isai, D. G., Kosa, E., Rajasingh, S., Kinsey, W., Neufeld, Z., & Rajasingh, J. (2017). Optical-flow based non-invasive analysis of cardiomyocyte contractility. Scientific Reports, 7(1), 10404. | en_US |
| dc.identifier.uri | http://hdl.handle.net/1808/26054 | |
| dc.description | A grant from the One-University Open Access Fund at the University of Kansas was used to defray the author's publication fees in this Open Access journal. The Open Access Fund, administered by librarians from the KU, KU Law, and KUMC libraries, is made possible by contributions from the offices of KU Provost, KU Vice Chancellor for Research & Graduate Studies, and KUMC Vice Chancellor for Research. For more information about the Open Access Fund, please see http://library.kumc.edu/authors-fund.xml. | en_US |
| dc.description.abstract | Characterization of cardiomyocyte beat patterns is needed for quality control of cells intended for surgical injection as well as to establish phenotypes in disease modeling or toxicity studies. Optical-flow based analysis of videomicroscopic recordings offer a manipulation-free and efficient characterization of contractile cycles, an important characteristics of cardiomyocyte phenotype. We demonstrate that by appropriate computational analysis of optical flow data one can identify distinct contractile centers and distinguish active cell contractility from passive elastic tissue deformations. Our proposed convergence measure correlates with myosin IIa immuno-localization and is capable to resolve contractile waves and their synchronization within maturing, unlabeled induced pluripotent stem cell-derived cardiomyocyte cultures. | en_US |
| dc.publisher | Nature Publishing Group | en_US |
| dc.rights | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.title | Optical-flow based non-invasive analysis of cardiomyocyte contractility | en_US |
| dc.type | Article | en_US |
| dc.identifier.doi | 10.1038/s41598-017-10094-7 | en_US |
| kusw.oaversion | Scholarly/refereed, publisher version | en_US |
| kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
| dc.rights.accessrights | openAccess | en_US |
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Except where otherwise noted, this item's license is described as: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
