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    Mechanistic investigations of matrix metalloproteinase-8 inhibition by metal abstraction peptide

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    Spencer_2016.pdf (1.268Mb)
    Issue Date
    2016-04-29
    Author
    Tucker, Jenifer K.
    McNiff, Michaela L.
    Ulapane, Sasanka B.
    Spencer, Paulette
    Laurence, Jennifer S.
    Berrie, Cindy L.
    Publisher
    AIP Publishing
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Rights
    The following article appeared in Biointerphases 11, 021006 (2016); https://doi.org/10.1116/1.4948340 and may be found at http://doi.org/10.1116/1.4948340.
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    Abstract
    The mechanism of matrix metalloproteinase-8 (MMP-8) inhibition was investigated using ellipsometric measurements of the interaction of MMP-8 with a surface bound peptide inhibitor, tether-metal abstraction peptide (MAP), bound to self-assembled monolayer films. MMP-8 is a collagenase whose activity and dysregulation have been implicated in a number of disease states, including cancer metastasis, diabetic neuropathy, and degradation of biomedical reconstructions, including dental restorations. Regulation of activity of MMP-8 and other matrix metalloproteinases is thus a significant, but challenging, therapeutic target. Strong inhibition of MMP-8 activity has recently been achieved via the small metal binding peptide tether-MAP. Here, the authors elucidate the mechanism of this inhibition and demonstrate that it occurs through the direct interaction of the MAP Tag and the Zn2+ binding site in the MMP-8 active site. This enhanced understanding of the mechanism of inhibition will allow the design of more potent inhibitors as well as assays important for monitoring critical MMP levels in disease states.
    URI
    http://hdl.handle.net/1808/25642
    DOI
    https://doi.org/10.1116/1.4948340
    Collections
    • Mechanical Engineering Scholarly Works [114]
    Citation
    Tucker, J. K., McNiff, M. L., Ulapane, S. B., Spencer, P., Laurence, J. S., & Berrie, C. L. (2016). Mechanistic investigations of matrix metalloproteinase-8 inhibition by metal abstraction peptide. Biointerphases, 11(2), 021006. http://doi.org/10.1116/1.4948340

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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