dc.contributor.author | Dixit, Namrata | |
dc.contributor.author | Settle, Jenifer K. | |
dc.contributor.author | Ye, Qiang | |
dc.contributor.author | Berrie, Cindy L. | |
dc.contributor.author | Spencer, Paulette | |
dc.contributor.author | Laurence, Jennifer S. | |
dc.date.accessioned | 2017-09-01T15:13:03Z | |
dc.date.available | 2017-09-01T15:13:03Z | |
dc.date.issued | 2015-02 | |
dc.identifier.citation | Dixit, N., Settle, J. K., Ye, Q., Berrie, C. L., Spencer, P., & Laurence, J. S. (2015). Grafting MAP peptide to dental polymer inhibits MMP-8 activity. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(2), 324–331. http://doi.org/10.1002/jbm.b.33205 | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/24884 | |
dc.description.abstract | Matrix metalloproteinases (MMPs) are a class of zinc and calcium-dependent endopeptidases responsible for degrading extracellular matrix (ECM) components. Their activity is critical for both normal biological function and pathological processes (Dejonckheere et al., Cytokine Growth Factor Rev 2011;22:73–81). In dental restorations, the release and subsequent acid activation of MMPs contributes to premature failure. In particular, MMP-8 accelerates degradation by cleaving the collagen matrix within the dentin substrate in incompletely infiltrated aged bonded dentin (Buzalaf et al., Adv Dent Res 2012;24:72–76), hastening the need for replacement of restorations. Therefore, development of a dental adhesive that better resists MMP-8 activity is of significant interest. We hypothesize that modification of the polymer surface with an inhibitor would disable MMP-8 activity. Here, we identify the metal abstraction peptide (MAP) as an inhibitor of MMP-8 and demonstrate that tethering MAP to methacrylate polymers effectively inhibits catalysis. Our findings indicate complete inhibition of MMP-8 is achievable using a grafting approach. This strategy has potential to improve longevity of dental adhesives and other polymers and enable rational design of a new generation of biocompatible materials. | en_US |
dc.publisher | Wiley | en_US |
dc.rights | This is the peer reviewed version of the following article: Dixit, N., Settle, J. K., Ye, Q., Berrie, C. L., Spencer, P., & Laurence, J. S. (2015). Grafting MAP peptide to dental polymer inhibits MMP-8 activity. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(2), 324–331. http://doi.org/10.1002/jbm.b.33205, which has been published in final form at http://doi.org/10.1002/jbm.b.33205. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en_US |
dc.subject | MMP-8 | en_US |
dc.subject | Dental adhesive | en_US |
dc.subject | Grafting | en_US |
dc.subject | Inhibitor | en_US |
dc.subject | Metal abstraction peptide | en_US |
dc.subject | Fluorescence assay | en_US |
dc.title | Grafting MAP peptide to dental polymer inhibits MMP-8 activity | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Dixit, Namrata | |
kusw.kuauthor | Settle, Jenifer K. | |
kusw.kuauthor | Ye, Qiang | |
kusw.kuauthor | Berrie, Cindy L. | |
kusw.kuauthor | Spencer, Paulette | |
kusw.kuauthor | Laurence, Jennifer S. | |
kusw.kudepartment | Bioengineering Research Center | en_US |
kusw.kudepartment | Chemistry | en_US |
kusw.kudepartment | Mechanical Engineering | en_US |
kusw.kudepartment | Pharmaceutical Chemistry | en_US |
dc.identifier.doi | 10.1002/jbm.b.33205 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.identifier.pmid | PMC5515473 | en_US |
dc.rights.accessrights | openAccess | |