Grafting MAP peptide to dental polymer inhibits MMP-8 activity

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Issue Date
2015-02Author
Dixit, Namrata
Settle, Jenifer K.
Ye, Qiang
Berrie, Cindy L.
Spencer, Paulette
Laurence, Jennifer S.
Publisher
Wiley
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
Rights
This is the peer reviewed version of the following article: Dixit, N., Settle, J. K., Ye, Q., Berrie, C. L., Spencer, P., & Laurence, J. S. (2015). Grafting MAP peptide to dental polymer inhibits MMP-8 activity. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(2), 324–331. http://doi.org/10.1002/jbm.b.33205, which has been published in final form at http://doi.org/10.1002/jbm.b.33205. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Show full item recordAbstract
Matrix metalloproteinases (MMPs) are a class of zinc and calcium-dependent endopeptidases responsible for degrading extracellular matrix (ECM) components. Their activity is critical for both normal biological function and pathological processes (Dejonckheere et al., Cytokine Growth Factor Rev 2011;22:73–81). In dental restorations, the release and subsequent acid activation of MMPs contributes to premature failure. In particular, MMP-8 accelerates degradation by cleaving the collagen matrix within the dentin substrate in incompletely infiltrated aged bonded dentin (Buzalaf et al., Adv Dent Res 2012;24:72–76), hastening the need for replacement of restorations. Therefore, development of a dental adhesive that better resists MMP-8 activity is of significant interest. We hypothesize that modification of the polymer surface with an inhibitor would disable MMP-8 activity. Here, we identify the metal abstraction peptide (MAP) as an inhibitor of MMP-8 and demonstrate that tethering MAP to methacrylate polymers effectively inhibits catalysis. Our findings indicate complete inhibition of MMP-8 is achievable using a grafting approach. This strategy has potential to improve longevity of dental adhesives and other polymers and enable rational design of a new generation of biocompatible materials.
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Citation
Dixit, N., Settle, J. K., Ye, Q., Berrie, C. L., Spencer, P., & Laurence, J. S. (2015). Grafting MAP peptide to dental polymer inhibits MMP-8 activity. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(2), 324–331. http://doi.org/10.1002/jbm.b.33205
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