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dc.contributor.authorSutherland, Amanda J.
dc.contributor.authorDetamore, Michael S.
dc.date.accessioned2017-06-20T19:01:06Z
dc.date.available2017-06-20T19:01:06Z
dc.date.issued2015-07
dc.identifier.citationSutherland, A. J. and Detamore, M. S. (2015), Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage. Macromol. Biosci., 15: 979–989. doi:10.1002/mabi.201400472en_US
dc.identifier.urihttp://hdl.handle.net/1808/24561
dc.descriptionThis is the peer reviewed version of the following article: Sutherland, A. J. and Detamore, M. S. (2015), Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage. Macromol. Biosci., 15: 979–989. doi:10.1002/mabi.201400472, which has been published in final form at http://doi.org/10.1002/mabi.201400472. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en_US
dc.description.abstractThe aim of this study was to fabricate mechanically functional microsphere-based scaffolds containing decellularized cartilage (DCC), with the hypothesis that this approach would induce chondrogenesis of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro. The DCC was derived from porcine articular cartilage and decellularized using a combination of physical and chemical methods. Four types of scaffolds were fabricated: Poly(D,L-lactic-co-glycolic acid) (PLGA) only (negative control), TGF-β encapsulated (positive control), PLGA surface coated with DCC, and DCC-encapsulated. These scaffolds were seeded with rBMSCs and cultured up to 6 weeks. The compressive modulus of the DCC-coated scaffolds prior to cell seeding was significantly lower than all other scaffold types. Gene expression was comparable between DCC-encapsulated and TGF-β encapsulated groups. Notably, DCC-encapsulated scaffolds contained 70% higher glycosaminoglyan (GAG) content and 85% more hydroxyproline compared to the TGF-β group at week 3 (with baseline levels subtracted out from acellular DCC scaffolds). Certainly bioactivity was demonstrated in eliciting a biosynthetic response from the cells with DCC, although true demonstration of chondrogenesis remained elusive under the prescribed conditions. Encapsulation of DCC appeared to lead to improved cell performance relative to coating with DCC, although this finding may be a dose-dependent observation. Overall, DCC introduced via microsphere-based scaffolds appears to be promising as a bioactive approach to cartilage regeneration, although additional studies will be required to conclusively demonstrate chondroinductivity.en_US
dc.publisherWileyen_US
dc.titleBioactive Microsphere-Based Scaffolds Containing Decellularized Cartilageen_US
dc.typeArticleen_US
kusw.kuauthorSutherland, Amanda
kusw.kuauthorDetamore, Michael
kusw.kudepartmentMechanical Engineeringen_US
dc.identifier.doi10.1002/mabi.201400472en_US
kusw.oaversionScholarly/refereed, author accepted manuscripten_US
kusw.oapolicyThis item meets KU Open Access policy criteria.en_US
dc.identifier.pmidPMC4504825en_US
dc.rights.accessrightsopenAccess


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