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    Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage

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    Detamore_2015.pdf (2.750Mb)
    Issue Date
    2015-07
    Author
    Sutherland, Amanda J.
    Detamore, Michael S.
    Publisher
    Wiley
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
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    Abstract
    The aim of this study was to fabricate mechanically functional microsphere-based scaffolds containing decellularized cartilage (DCC), with the hypothesis that this approach would induce chondrogenesis of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro. The DCC was derived from porcine articular cartilage and decellularized using a combination of physical and chemical methods. Four types of scaffolds were fabricated: Poly(D,L-lactic-co-glycolic acid) (PLGA) only (negative control), TGF-β encapsulated (positive control), PLGA surface coated with DCC, and DCC-encapsulated. These scaffolds were seeded with rBMSCs and cultured up to 6 weeks. The compressive modulus of the DCC-coated scaffolds prior to cell seeding was significantly lower than all other scaffold types. Gene expression was comparable between DCC-encapsulated and TGF-β encapsulated groups. Notably, DCC-encapsulated scaffolds contained 70% higher glycosaminoglyan (GAG) content and 85% more hydroxyproline compared to the TGF-β group at week 3 (with baseline levels subtracted out from acellular DCC scaffolds). Certainly bioactivity was demonstrated in eliciting a biosynthetic response from the cells with DCC, although true demonstration of chondrogenesis remained elusive under the prescribed conditions. Encapsulation of DCC appeared to lead to improved cell performance relative to coating with DCC, although this finding may be a dose-dependent observation. Overall, DCC introduced via microsphere-based scaffolds appears to be promising as a bioactive approach to cartilage regeneration, although additional studies will be required to conclusively demonstrate chondroinductivity.
    Description
    This is the peer reviewed version of the following article: Sutherland, A. J. and Detamore, M. S. (2015), Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage. Macromol. Biosci., 15: 979–989. doi:10.1002/mabi.201400472, which has been published in final form at http://doi.org/10.1002/mabi.201400472. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
    URI
    http://hdl.handle.net/1808/24561
    DOI
    https://doi.org/10.1002/mabi.201400472
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    • Mechanical Engineering Scholarly Works [91]
    Citation
    Sutherland, A. J. and Detamore, M. S. (2015), Bioactive Microsphere-Based Scaffolds Containing Decellularized Cartilage. Macromol. Biosci., 15: 979–989. doi:10.1002/mabi.201400472

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    KU Libraries
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    785-864-8983

    KU Libraries
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    Lawrence, KS 66045
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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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