PLGA-chitosan/PLGA-alginate Nanoparticle Blends as Biodegradable Colloidal Gels for Seeding Human Umbilical Cord Mesenchymal Stem Cells
View/ Open
Issue Date
2011-03-01Author
Wang, Qun
Jamal, Syed A.
Detamore, Michael S.
Berkland, Cory J.
Publisher
Wiley
Type
Article
Article Version
Scholarly/refereed, author accepted manuscript
Rights
This is the peer reviewed version of the following article: Wang, Q., Jamal, S., Detamore, M. S. and Berkland, C. (2011), PLGA-chitosan/PLGA-alginate nanoparticle blends as biodegradable colloidal gels for seeding human umbilical cord mesenchymal stem cells. J. Biomed. Mater. Res., 96A: 520–527. doi:10.1002/jbm.a.33000, which has been published in final form at http://doi.org/10.1002/jbm.a.33000. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Metadata
Show full item recordAbstract
The natural polymers chitosan and alginate represent an attractive material choice for biodegradable inplants. These were used as coating materials to make positively and negatively charged PLGA nanoparticles, respectively. After blending at total solids concentration >10% wt/vol, these oppositely charged nanoparticles yielded a cohesive colloidal gel. Electrostatic forces between oppositely charged nanoparticles produced a stable 3-D porous network that may be extruded or molded to the desired shape. This high concentration colloidal system demonstrated shear-thinning behavior due to the disruption of interparticle interactions. Once the external force was removed, the cohesive property of the colloidal gel was recovered. Scanning electron micrographs of dried colloidal networks revealed an organized, 3-D microporous structure. Rheological studies were employed to probe the differences in plasticity and shear sensitivity of colloidal gels. Viability tests of hUCMSCs seeded on the colloidal gels also demonstrated the negligible cytotoxicity of the materials. All the results indicated the potential application of the biodegradable colloidal gels as an injectable scaffold in tissue engineering and drug release.
Collections
Citation
Wang, Q., Jamal, S., Detamore, M. S. and Berkland, C. (2011), PLGA-chitosan/PLGA-alginate nanoparticle blends as biodegradable colloidal gels for seeding human umbilical cord mesenchymal stem cells. J. Biomed. Mater. Res., 96A: 520–527. doi:10.1002/jbm.a.33000
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.