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    NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

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    Wang_ACS_2010.pdf (3.163Mb)
    Issue Date
    2010-05-18
    Author
    Wang, Yu
    Nordhues, Bryce Andrew
    Zhong, Dalian
    De Guzman, Roberto N.
    Publisher
    ACS
    Type
    Article
    Article Version
    Scholarly/refereed, author accepted manuscript
    Rights
    Copyright © 2010 American Chemical Society
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    Abstract
    Salmonella and Shigella bacteria require the type III secretion system (T3SS) to inject virulence proteins into their hosts and initiate infections. The tip proteins SipD and IpaD are critical components of the Salmonella and Shigella T3SS, respectively. Recently, SipD and IpaD have been shown to interact with bile salts, which are enriched in the intestines, and are hypothesized to act as environmental sensors for these enteric pathogens. Bile salts activate the Shigella T3SS but repress the Salmonella T3SS, and the mechanism of this differing response to bile salts is poorly understood. Further, how SipD binds to bile salts is currently unknown. Computer modeling predicted that IpaD binds the bile salt deoxycholate in a cleft formed by the N-terminal domain and the long central coiled coil of IpaD. Here, we used NMR methods to determine which SipD residues are affected by the interaction with the bile salts deoxycholate, chenodeoxycholate and taurodeoxcholate. The bile salts perturbed nearly the same set of SipD residues, however, the largest chemical shift perturbations occurred away from what was predicted for the bile salt binding site in IpaD. Our NMR results indicate that that bile salt interaction of SipD will be different from what was predicted for IpaD, suggesting a possible mechanism for the differing response of Salmonella and Shigella to bile salts.
    URI
    http://hdl.handle.net/1808/23705
    DOI
    https://doi.org/10.1021/bi100335u
    Collections
    • Molecular Biosciences Scholarly Works [581]
    Citation
    Wang, Y., Nordhues, B., Zhong, D., & De Guzman, R. N. (2010). NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts. Biochemistry, 49(19), 4220–4226. http://doi.org/10.1021/bi100335u

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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