dc.contributor.author | Li, Ling | |
dc.contributor.author | Tang, Wenhua | |
dc.contributor.author | Wu, Xiaoqing | |
dc.contributor.author | Karnak, David | |
dc.contributor.author | Meng, Xiaojie | |
dc.contributor.author | Thompson, Rachel | |
dc.contributor.author | Hao, Xinbao | |
dc.contributor.author | Li, Yongmin | |
dc.contributor.author | Qiao, Xiaotan T. | |
dc.contributor.author | Lin, Jiayuh | |
dc.contributor.author | Fuchs, James R. | |
dc.contributor.author | Simeone, Diane M. | |
dc.contributor.author | Chen, Zhi-Nan | |
dc.contributor.author | Lawrence, Theodore S. | |
dc.contributor.author | Xu, Liang | |
dc.date.accessioned | 2017-02-27T21:31:10Z | |
dc.date.available | 2017-02-27T21:31:10Z | |
dc.date.issued | 2014-12-15 | |
dc.identifier.citation | Li, L., W. Tang, X. Wu, D. Karnak, X. Meng, R. Thompson, X. Hao, Y. Li, X. T. Qiao, J. Lin, J. Fuchs, D. M. Simeone, Z.-N. Chen, T. S. Lawrence, and L. Xu. "HAb18G/CD147 Promotes PSTAT3-Mediated Pancreatic Cancer Development via CD44s." Clinical Cancer Research 19.24 (2013): 6703-715. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/23274 | |
dc.description.abstract | PurposeSTAT3 plays a critical role in initiation and progression of pancreatic cancer. However, therapeutically targeting STAT3 is failure in clinic. We previously identified HAb18G/CD147 as an effective target for cancer treatment. In this study, we aimed to investigate potential role of HAb18G/CD147 in STAT3-involved pancreatic tumorigenesis in vitro and in vivo.Experimental DesignThe expression of HAb18G/CD147, pSTAT3 and CD44s were determined in tissue microarrays. The tumorigenic function and molecular signaling mechanism of HAb18G/CD147 was assessed by in vitro cellular and clonogenic growth, reporter assay, immunoblot, immunofluorescence staining, immunoprecipitation, and in vivo tumor formationusing loss or gain-of-function strategies.ResultsHighly expressed HAb18G/CD147 promoted cellular and clonogenic growth in vitro and tumorigenicity in vivo. CyPA, a ligand of CD147, stimulated STAT3 phosphorylation and its downstream genes cyclin D1/survivin through HAb18G/CD147 dependent mechanisms. HAb18G/CD147 was associated and co-localized with cancer stem cell marker CD44s in lipid rafts. The inhibitors of STAT3 and survivin, as well as CD44s neutralizing antibodies suppressed the HAb18G/CD147-induced cell growth. High HAb18G/CD147 expression in pancreatic cancer was significantly correlated with the poor tumor differentiation, and the high co-expression of HAb18G/CD147-CD44s-STAT3 associated with poor survival of patients with pancreatic cancer.ConclusionsWe identified HAb18G/CD147 as a novel upstream activator of STAT3 via interacts with CD44s and plays a critical role in the development of pancreatic cancer. The data suggest HAb18G/CD147 could be a promising therapeutic target for highly aggressive pancreatic cancer and a surrogate marker in the STAT3-targeted molecular therapies. | en_US |
dc.publisher | American Association for Cancer Research | en_US |
dc.relation.isversionof | http://clincancerres.aacrjournals.org/content/19/24/6703.full-text.pdf | en_US |
dc.subject | Pancreatic cancer | en_US |
dc.subject | HAb18G/CD147 | en_US |
dc.subject | CD44 | en_US |
dc.subject | STAT3 | en_US |
dc.subject | Tumor development | en_US |
dc.title | HAb18G/CD147 Promotes pSTAT3-Mediated Pancreatic Cancer Development via CD44s | en_US |
dc.type | Article | en_US |
kusw.kuauthor | Wu, Xiaoqing | |
kusw.kudepartment | Higuchi Biosciences Center | en_US |
dc.identifier.doi | 10.1158/1078-0432.CCR-13-0621 | en_US |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | en_US |
kusw.oapolicy | This item meets KU Open Access policy criteria. | en_US |
dc.rights.accessrights | openAccess | |