Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription
dc.contributor.author | Kayal, Ehsan | |
dc.contributor.author | Bentlage, Bastian | |
dc.contributor.author | Cartwright, Paulyn | |
dc.contributor.author | Yanagihara, Angel A. | |
dc.contributor.author | Lindsay, Dhugal | |
dc.contributor.author | Hopcroft, Russell R. | |
dc.contributor.author | Collins, Allen G. | |
dc.date.accessioned | 2016-01-08T20:02:19Z | |
dc.date.available | 2016-01-08T20:02:19Z | |
dc.date.issued | 2015-11-19 | |
dc.identifier.citation | Kayal, Ehsan, Bastian Bentlage, Paulyn Cartwright, Angel A. Yanagihara, Dhugal J. Lindsay, Russell R. Hopcroft, and Allen G. Collins. "Phylogenetic Analysis of Higher-level Relationships within Hydroidolina (Cnidaria: Hydrozoa) Using Mitochondrial Genome Data and Insight into Their Mitochondrial Transcription." PeerJ 3 (2015): n. pag. http://dx.doi.org/10.7717/peerj.1403. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/19743 | |
dc.description.abstract | Hydrozoans display the most morphological diversity within the phylum Cnidaria. While recent molecular studies have provided some insights into their evolutionary history, sister group relationships remain mostly unresolved, particularly at mid-taxonomic levels. Specifically, within Hydroidolina, the most speciose hydrozoan subclass, the relationships and sometimes integrity of orders are highly unsettled. Here we obtained the near complete mitochondrial sequence of twenty-six hydroidolinan hydrozoan species from a range of sources (DNA and RNA-seq data, long-range PCR). Our analyses confirm previous inference of the evolution of mtDNA in Hydrozoa while introducing a novel genome organization. Using RNA-seq data, we propose a mechanism for the expression of mitochondrial mRNA in Hydroidolina that can be extrapolated to the other medusozoan taxa. Phylogenetic analyses using the full set of mitochondrial gene sequences provide some insights into the order-level relationships within Hydroidolina, including siphonophores as the first diverging clade, a well-supported clade comprised of Leptothecata-Filifera III–IV, and a second clade comprised of Aplanulata-Capitata s.s.-Filifera I–II. Finally, we describe our relatively inexpensive and accessible multiplexing strategy to sequence long-range PCR amplicons that can be adapted to most high-throughput sequencing platforms. | en_US |
dc.publisher | PeerJ | en_US |
dc.rights | This is an open access article, free of all copyright, made available under the Creative Commons Public Domain Dedication. This work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. | |
dc.rights.uri | https://creativecommons.org/publicdomain/zero/1.0/ | |
dc.subject | Phylomitogenomics | en_US |
dc.subject | Low-cost multiplexing library preparation | en_US |
dc.subject | Filifera | en_US |
dc.subject | Siphonophora | en_US |
dc.subject | Capitata | en_US |
dc.subject | Aplanulata | en_US |
dc.subject | Leptothecata | en_US |
dc.subject | Mitochondrial gene expression | en_US |
dc.title | Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription | en_US |
dc.type | Article | |
kusw.kuauthor | Cartwright, Paulyn | |
kusw.kudepartment | Ecology & Evolutionary Biology | en_US |
dc.identifier.doi | 10.7717/peerj.1403 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as: This is an open access article, free of all copyright, made available under the Creative Commons Public Domain Dedication. This work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.