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    Multiplex DNA Typing of Short-Tandem-Repeat Loci on the Y Chromosome

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    Issue Date
    1997-08
    Author
    Redd, Alan J.
    Clifford, Stephanie L.
    Stoneking, Mark
    Publisher
    De Gruyter
    Type
    Article
    Article Version
    Scholarly/refereed, publisher version
    Metadata
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    Abstract
    To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandemrepeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determined with an automated DNA sequencer. We typed 162 males from three U.S. populations (African-Americans, European-Americans and Hispanics) and found that the haplotype diversities range from 0.920 to 0.969. This quadruplex system provides a facile means of genotyping these Y chromosome STRs, and should be useful in population genetic and forensic applications.
    Description
    This is the published version. Copyright 1997 de Gruyter.
    URI
    http://hdl.handle.net/1808/17984
    Collections
    • Anthropology Scholarly Works [206]
    Citation
    Redd, Alan J., Stephanie L. Clifford, and Mark Stoneking. "Multiplex DNA Typing of Short-tandem-repeat Loci on the Y Chromosome." Biological Chemistry 378.8 (1997): 923-27.

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    Contact KU ScholarWorks
    785-864-8983
    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    785-864-8983

    KU Libraries
    1425 Jayhawk Blvd
    Lawrence, KS 66045
    Image Credits
     

     

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