Multiplex DNA Typing of Short-Tandem-Repeat Loci on the Y Chromosome

View/ Open
Issue Date
1997-08Author
Redd, Alan J.
Clifford, Stephanie L.
Stoneking, Mark
Publisher
De Gruyter
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
To facilitate evolutionary and forensic studies of DNA
polymorphisms on the Y chromosome, we devised a
multiplex amplification procedure for short-tandemrepeat
(STR) loci. Four tetranucleotide STR loci
(DYS19, DYS390, DYS391, and DYS393) were simultaneously
amplified with FAM-labeled primers and
genotypes were determined with an automated DNA
sequencer. We typed 162 males from three U.S. populations
(African-Americans, European-Americans and
Hispanics) and found that the haplotype diversities
range from 0.920 to 0.969. This quadruplex system
provides a facile means of genotyping these Y chromosome
STRs, and should be useful in population
genetic and forensic applications.
Description
This is the published version. Copyright 1997 de Gruyter.
Collections
Citation
Redd, Alan J., Stephanie L. Clifford, and Mark Stoneking. "Multiplex DNA Typing of Short-tandem-repeat Loci on the Y Chromosome." Biological Chemistry 378.8 (1997): 923-27.
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.