dc.contributor.author | Karakurum, M. | |
dc.contributor.author | Shreeniwas, R. | |
dc.contributor.author | Chen, Jing Xian | |
dc.contributor.author | Pinsky, David J. | |
dc.contributor.author | Yan, Shirley ShiDu | |
dc.contributor.author | Anderson, M. | |
dc.contributor.author | Sunouchi, K. | |
dc.contributor.author | Major, J. | |
dc.contributor.author | Hamilton, T. | |
dc.contributor.author | Kuwabara, Keisuke | |
dc.date.accessioned | 2015-05-28T14:47:03Z | |
dc.date.available | 2015-05-28T14:47:03Z | |
dc.date.issued | 1994-04-01 | |
dc.identifier.citation | Karakurum, M., R. Shreeniwas, J. Chen, D. Pinsky, S. D. Yan, M. Anderson, K. Sunouchi, J. Major, T. Hamilton, and K. Kuwabara. "Hypoxic Induction of Interleukin-8 Gene Expression in Human Endothelial Cells." Journal of Clinical Investigation J. Clin. Invest. 93.4 (1994): 1564-570. http://dx.doi.org/10.1172/JCI117135. | en_US |
dc.identifier.uri | http://hdl.handle.net/1808/17860 | |
dc.description | This is the published version. Copyright 1994 American Society for Clinical Investigation. | en_US |
dc.description.abstract | Because leukocyte-mediated tissue damage is an important component of the pathologic picture in ischemia/reperfusion, we have sought mechanisms by which PMNs are directed into hypoxic tissue. Incubation of human endothelial cells (ECs) in hypoxia, PO2 approximately 14-18 Torr, led to time-dependent release of IL-8 antigen into the conditioned medium; this was accompanied by increased chemotactic activity for PMNs, blocked by antibody to IL-8. Production of IL-8 by hypoxic ECs occurred concomitantly with both increased levels of IL-8 mRNA, based on polymerase chain reaction analysis, and increased IL-8 transcription, based on nuclear run-on assays. Northern analysis of mRNA from hypoxic ECs also demonstrated increased levels of mRNA for macrophage chemotactic protein-1, another member of the chemokine superfamily of proinflammatory cytokines. IL-8 gene induction was associated with the presence of increased binding activity in nuclear extracts from hypoxic ECs for the NF-kB site. Studies with human umbilical vein segments exposed to hypoxia also demonstrated increased elaboration of IL-8 antigen compared with normoxic controls. In mice exposed to hypoxia (PO2 approximately 30-40 Torr), there was increased pulmonary leukostasis, as evidenced by increased myeloperoxidase activity in tissue homogenates. In parallel, increased levels of transcripts for IP-10, a murine homologue in the chemokine family related to IL-8, were observed in hypoxic lung tissue. Taken together, these data suggest that hypoxia constitutes a stimulus for leukocyte chemotaxis and tissue leukostasis. | en_US |
dc.publisher | American Society for Clinical Investigation | en_US |
dc.title | Hypoxic induction of interleukin-8 gene expression in human endothelial cells. | en_US |
dc.type | Article | |
kusw.kuauthor | Yan, Shirley ShiDu | |
kusw.kudepartment | Pharmacology & Toxicology | en_US |
dc.identifier.doi | 10.1172/JCI117135 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |