dc.contributor.author | Khan, Liakot A. | |
dc.contributor.author | Zhang, Hongjie | |
dc.contributor.author | Abraham, Nessy | |
dc.contributor.author | Sun, Lei | |
dc.contributor.author | Fleming, John T. | |
dc.contributor.author | Buechner, Matthew | |
dc.contributor.author | Hall, David H. | |
dc.contributor.author | Gobel, Verena | |
dc.date.accessioned | 2014-08-11T15:33:30Z | |
dc.date.available | 2014-08-11T15:33:30Z | |
dc.date.issued | 2013-02-01 | |
dc.identifier.citation | Khan, Liakot A., Zhang, Hongjie., Abraham, Nessy., Sun, Lei., Fleming, John T., Buechner, Matthew., Hall, David H., Gobel, Verna. "Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux." Nature Cell Biology. January 20, 2013. http:dx.doi.org/10.1038/ncb2656. | |
dc.identifier.uri | http://hdl.handle.net/1808/14907 | |
dc.description | This is the author's accepted manuscript, it is also available here, http:dx.doi.org/10.1038/ncb2656. | |
dc.description.abstract | Many unicellular tubes such as capillaries form lumens intracellularly, a process that is not well understood. Here we show that the cortical membrane organizer ERM-1 is required to expand the intracellular apical/lumenal membrane and its actin undercoat during single-cell Caenorhabditis elegans excretory canal morphogenesis. We characterize AQP-8, identified in an ERM-1-overexpression (ERM-1[++]) suppressor screen, as a canalicular aquaporin that interacts with ERM-1 in lumen extension in a mercury-sensitive manner, implicating water-channel activity. AQP-8 is transiently recruited to the lumen by ERM-1, co-localizing in peri-lumenal cuffs interspaced along expanding canals. An ERM-1[++]-mediated increase in the number of lumen-associated canaliculi is reversed by AQP-8 depletion. We propose that the ERM-1/AQP-8 interaction propels lumen extension by translumenal flux, suggesting a direct morphogenetic effect of water-channel-regulated fluid pressure. | |
dc.publisher | Nature Publishing Group | |
dc.title | Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux | |
dc.type | Article | |
kusw.kuauthor | Buechner, Matthew | |
kusw.kudepartment | Molecular Biosciences | |
dc.identifier.doi | 10.1038/ncb2656 | |
dc.identifier.orcid | https://orcid.org/0000-0002-2103-9399 | |
kusw.oaversion | Scholarly/refereed, author accepted manuscript | |
kusw.oapolicy | This item meets KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess | |