All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote
dc.contributor.author | Gong, Wei J. | |
dc.contributor.author | McKim, Kim S. | |
dc.contributor.author | Hawley, R. Scott | |
dc.date.accessioned | 2014-03-18T21:28:39Z | |
dc.date.available | 2014-03-18T21:28:39Z | |
dc.date.issued | 2005-11-18 | |
dc.identifier.citation | Gong, W. J., McKim, K. S., & Hawley, R. S. (2005). All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote. PLoS Genet, 1(5). http://dx.doi.org/10.1371/journal.pgen.0010067 | |
dc.identifier.uri | http://hdl.handle.net/1808/13245 | |
dc.description.abstract | The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP: lacO system to visualize the effects of FM7 on meiotic pairing, synapsis, and double-strand break formation in Drosophila oocytes. Surprisingly, the analysis of meiotic pairing and synapsis for three lacO reporter couplets in FM7/X heterozygotes revealed they are paired and synapsed during zygotene/pachytene in 70%–80% of oocytes. Moreover, the regions defined by these lacO couplets undergo double-strand break formation at normal frequency. Thus, even complex aberration heterozygotes usually allow high frequencies of meiotic pairing, synapsis, and double-strand break formation in Drosophila oocytes. However, the frequencies of failed pairing and synapsis were still 1.5- to 2-fold higher than were observed for corresponding regions in oocytes with two normal sequence X chromosomes, and this effect was greatest near a breakpoint. We propose that heterozygosity for breakpoints creates a local alteration in synaptonemal complex structure that is propagated across long regions of the bivalent in a fashion analogous to chiasma interference, which also acts to suppress crossing over. | |
dc.publisher | Public Library of Science | |
dc.rights | ©2005 Gong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Choromosome pairs | |
dc.subject | Chromosome structure and function | |
dc.subject | Chromosomes | |
dc.subject | Drosophila melanogaster | |
dc.subject | Heterozygosity | |
dc.subject | Oocytes | |
dc.subject | Synapsis | |
dc.subject | Xchromosomes | |
dc.title | All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote | |
dc.type | Article | |
kusw.kuauthor | Hawley, R. Scott | |
kusw.kudepartment | Molecular Biosciences | |
kusw.oastatus | na | |
dc.identifier.doi | 10.1371/journal.pgen.0010067 | |
kusw.oaversion | Scholarly/refereed, publisher version | |
kusw.oapolicy | This item does not meet KU Open Access policy criteria. | |
dc.rights.accessrights | openAccess |
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Except where otherwise noted, this item's license is described as: ©2005 Gong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.