Thumbnail Image
Publication

Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements

Armendariz, Kevin P.
; 
Dunn, Robert C.
Citations
Altmetric:
Abstract
Single molecule fluorescence measurements are used to probe the effects of GM1 in DPPC monolayers. Langmuir-Blodgett films of GM1 and DPPC were doped with ~10−8 mol% of the fluorescent lipid probe, BODIPY-PC, and transferred onto glass substrates at 23 mN/m. As shown previously, the individual orientation of each BODIPY-PC probe in the membrane can be measured using defocused polarized total internal reflection fluorescence microscopy, revealing changes in film properties at the molecular level. Here, BODIPY-PC tilt angle histograms are used to characterize the effects of GM1 in DPPC films from 0.05 mol% to 100 mol% GM1. At high GM1 levels (>5 mol% GM1), trends in the single molecule measurements agree with previous bulk measurements showing the turnover from condensing to expanding influence of GM1 at ~20 mol%, thus validating the single molecule approach. At biologically relevant, low concentrations of GM1 (<5 mol% GM1), where bulk fluorescence measurements are less informative, the single molecule measurements reveal a marked influence of GM1 on film properties. The addition of trace amounts of GM1 to DPPC films leads to an expansion of the film which continues to 0.10 mol% GM1, above which the trend reverses and the condensing effect previously noted is observed.
Description
Date
2013-07-03
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Collections
Chemistry Scholarly Works
Show all metadata
Files
Thumbnail Image
Armendariz_2013.pdf
Adobe PDF, 1.74 MB
Research Projects
Organizational Units
Journal Issue
Keywords
GM1, DPPC, Fluorescence, Partitioning, Langmuir-Blodgett films, Total internal reflection
Citation
Armendariz, K. P., & Dunn, R. C. (2013). Ganglioside Influence on Phospholipid Films Investigated with Single Molecule Fluorescence Measurements. The Journal of Physical Chemistry. B, 117(26), 7959–7966. http://doi.org/10.1021/jp405312a
URI
https://hdl.handle.net/1808/23850
DOI
10.1021/jp405312a
Embedded videos