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Kinetic Mechanism of DNA Translocation by the RSC Molecular Motor
Eastlund, Allen Malik, Shuja Shafi Fischer, Christopher J.
Eastlund, Allen
Malik, Shuja Shafi
Fischer, Christopher J.
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Abstract
ATP-dependent nucleosome repositioning by chromatin remodeling enzymes requires the translocation of these enzymes along the nucleosomal DNA. Using a fluorescence stopped-flow assay we monitored DNA translocation by a minimal RSC motor and through global analysis of these time courses we have determined that this motor has a macroscopic translocation rate of 2.9 bp/s with a step size of 1.24 bp. From the complementary quantitative analysis of the associated time courses of ATP consumption during DNA translocation we have determined that this motor has an efficiency of 3.0 ATP/bp, which is slightly less that the efficiency observed for several genetically related DNA helicases and which likely results from random pausing by the motor during translocation. Nevertheless, this motor is able to exert enough force during translocation to displace streptavidin from biotinylated DNA. Taken together these results are the necessary first step for quantifying both the role of DNA translocation in nucleosome repositioning by RSC and the efficiency at which RSC couples ATP binding and hydrolysis to nucleosome repositioning.
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2013-02-09
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Elsevier
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fischer_kinetic.pdf
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Chromatin remodeling enzyme, RSC, DNA translocation, Energy transduction, Kinetic modeling
Citation
Eastlund, Allen, Shuja Shafi Malik, and Christopher J. Fischer. "Kinetic Mechanism of DNA Translocation by the RSC Molecular Motor." Archives of Biochemistry and Biophysics 532.2 (2013): 73-83.