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Sequence composition and environment effects on residue fluctuations in protein structures
Ruvinsky, Anatoly M. Vakser, Ilya A.
Ruvinsky, Anatoly M.
Vakser, Ilya A.
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Abstract
Structure fluctuations in proteins affect a broad range of cell phenomena, including stability of proteins and their fragments, allosteric transitions, and energy transfer. This study presents a statistical-thermodynamic analysis of relationship between the sequence composition and the distribution of residue fluctuations in protein-protein complexes. A one-node-per-residue elastic network model accounting for the nonhomogeneous protein mass distribution and the interatomic interactions through the renormalized inter-residue potential is developed. Two factors, a protein mass distribution and a residue environment, were found to determine the scale of residue fluctuations. Surface residues undergo larger fluctuations than core residues in agreement with experimental observations. Ranking residues over the normalized scale of fluctuations yields a distinct classification of amino acids into three groups: (i) highly fluctuating-Gly, Ala, Ser, Pro, and Asp, (ii) moderately fluctuating-Thr, Asn, Gln, Lys, Glu, Arg, Val, and Cys, and (iii) weakly fluctuating-Ile, Leu, Met, Phe, Tyr, Trp, and His. The structural instability in proteins possibly relates to the high content of the highly fluctuating residues and a deficiency of the weakly fluctuating residues in irregular secondary structure elements (loops), chameleon sequences, and disordered proteins. Strong correlation between residue fluctuations and the sequence composition of protein loops supports this hypothesis. Comparing fluctuations of binding site residues (interface residues) with other surface residues shows that, on average, the interface is more rigid than the rest of the protein surface and Gly, Ala, Ser, Cys, Leu, and Trp have a propensity to form more stable docking patches on the interface. The findings have broad implications for understanding mechanisms of protein association and stability of proteinstructures.
Description
This is the published version, also available here: http://dx.doi.org/10.1063/1.3498743.
Date
2010-10-15
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American Institute of Physics
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Keywords
Proteins, Acids, Binding sites, Secondary Structure, Crystal structure
Citation
Ruvinsky, Anatoly M. & Vakser Ilya A. "Sequence composition and environment effects on residue fluctuations in protein structures." J. Chem. Phys. 133, 155101 (2010); http://dx.doi.org/10.1063/1.3498743