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Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production
Soukup, Alexandra A. Chiang, Yi-Ming Bok, Jin Woo Reyes-Dominguez, Yazmid Oakley, Berl R. Wang, Clay C. C. Strauss, Joseph Keller, Nancy P.
Soukup, Alexandra A.
Chiang, Yi-Ming
Bok, Jin Woo
Reyes-Dominguez, Yazmid
Oakley, Berl R.
Wang, Clay C. C.
Strauss, Joseph
Keller, Nancy P.
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Abstract
Regulation of secondary metabolite (SM) gene clusters in Aspergillus nidulans has been shown to occur through cluster specific transcription factors or through global regulators of chromatin structure such as histone methyltransferases, histone deacetylases, or the putative methyltransferase LaeA. A multi-copy suppressor screen for genes capable of returning SM production to the SM deficient ΔlaeA mutant resulted in identification of the essential histone acetyltransferase EsaA, able to complement an esa1 deletion in Saccharomyces cereviseae. Here we report that EsaA plays a novel role in SM cluster activation through histone 4 lysine 12 (H4K12) acetylation in four examined SM gene clusters (sterigmatocystin, penicillin, terrequinone, and orsellinic acid), in contrast to no increase in H4K12 acetylation of the housekeeping tubA promoter. This augmented SM cluster acetylation requires LaeA for full effect and correlates with both increased transcript levels and metabolite production relative to wild type. H4K12 levels may thus represent a unique indicator of relative production potential, notably of SMs.
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This is the peer reviewed version of the following article: Soukup, A. A., Chiang, Y.-M., Bok, J. W., Reyes-Dominguez, Y., Oakley, B. R., Wang, C. C. C., Strauss, J. and Keller, N. P. (2012), Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production. Molecular Microbiology, 86: 314–330. doi:10.1111/j.1365-2958.2012.08195.x, which has been published in final form at http://doi.org/10.1111/j.1365-2958.2012.08195.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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2012-10
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Wiley
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Soukup, A. A., Chiang, Y.-M., Bok, J. W., Reyes-Dominguez, Y., Oakley, B. R., Wang, C. C. C., Strauss, J. and Keller, N. P. (2012), Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production. Molecular Microbiology, 86: 314–330. doi:10.1111/j.1365-2958.2012.08195.x