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Oligomerization-dependent Regulation of Motility and Morphogenesis by the Collagen XVIII NC1/Endostatin Domain
Kuo, Calvin J. LaMontagne, Kenneth R. Garcia-Cardeña, Guillermo Ackley, Brian D. Kalman, Daniel Park, Susan Christofferson, Rolf Kamihara, Junne Ding, Yuan-Hua Lo, Kin-Ming
Kuo, Calvin J.
LaMontagne, Kenneth R.
Garcia-Cardeña, Guillermo
Ackley, Brian D.
Kalman, Daniel
Park, Susan
Christofferson, Rolf
Kamihara, Junne
Ding, Yuan-Hua
Lo, Kin-Ming
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Abstract
Collagen XVIII (c18) is a triple helical endothelial/epithelial basement membrane protein whose noncollagenous (NC)1 region trimerizes a COOH-terminal endostatin (ES) domain conserved in vertebrates, Caenorhabditis elegans and Drosophila. Here, the c18 NC1 domain functioned as a motility-inducing factor regulating the extracellular matrix (ECM)-dependent morphogenesis of endothelial and other cell types. This motogenic activity required ES domain oligomerization, was dependent on rac, cdc42, and mitogen-activated protein kinase, and exhibited functional distinction from the archetypal motogenic scatter factors hepatocyte growth factor and macrophage stimulatory protein. The motility-inducing and mitogen-activated protein kinase–stimulating activities of c18 NC1 were blocked by its physiologic cleavage product ES monomer, consistent with a proteolysis-dependent negative feedback mechanism. These data indicate that the collagen XVIII NC1 region encodes a motogen strictly requiring ES domain oligomerization and suggest a previously unsuspected mechanism for ECM regulation of motility and morphogenesis.
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This is the publisher's version, also available electronically from http://jcb.rupress.org/content/152/6/1233.
Date
2001-03-19
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The Rockefeller University Press
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Keywords
Collagen XVIII, endostatin, Motility, Morphogenesis, Extracellular matrix
Citation
Kuo, Calvin J. 2001. "Oligomerization-dependent Regulation of Motility and Morphogenesis by the Collagen XVIII NC1/Endostatin Domain." Journal of Cell Biology, v. 1152, pp. 1233. http://www.dx.doi.org/10.1083/jcb.152.6.1233