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Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations
Hodge, Jacob G. Robinson, Jennifer L. Mellott, Adam J.
Hodge, Jacob G.
Robinson, Jennifer L.
Mellott, Adam J.
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Abstract
Aim: To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system.
Methods: Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population.
Results: The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers.
Conclusion: This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies.
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Date
2022-10-12
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Future Science Group
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Keywords
Cell culture, Hydrogels, Regenerative medicine, Stem cells, Tissue engineering
Citation
Hodge, J. G., Robinson, J. L., & Mellott, A. J. (2023). Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations. Regenerative medicine, 18(1), 23–36. https://doi.org/10.2217/rme-2022-0140