Loading...
Drosophila coracle, a Member of the Protein 4.1 Superfamily, Has Essential Structural Functions in the Septate Junctions and Developmental Functions in Embryonic and Adult Epithelial Cells
Lamb, Rebecca S. Schweizer, Liang Fehon, Richard G. Ward, Robert E., IV
Lamb, Rebecca S.
Schweizer, Liang
Fehon, Richard G.
Ward, Robert E., IV
Citations
Altmetric:
Abstract
Although extensively studied biochemically, members of the Protein 4.1 superfamily have not been as well characterized genetically. Studies of coracle, a DrosophilaProtein 4.1 homologue, provide an opportunity to examine the genetic functions of this gene family. coracle was originally identified as a dominant suppressor ofEgfr Elp, a hypermorphic form of theDrosophila Epidermal growth factor receptor gene. In this article, we present a phenotypic analysis ofcoracle, one of the first for a member of the Protein 4.1 superfamily. Screens for new coracle alleles confirm the null coracle phenotype of embryonic lethality and failure in dorsal closure, and they identify additional defects in the embryonic epidermis and salivary glands. Hypomorphiccoracle alleles reveal functions in many imaginal tissues. Analysis of coracle mutant cells indicates that Coracle is a necessary structural component of the septate junction required for the maintenance of the transepithelial barrier but is not necessary for apical–basal polarity, epithelial integrity, or cytoskeletal integrity. In addition, coracle phenotypes suggest a specific role in cell signaling events. Finally, complementation analysis provides information regarding the functional organization of Coracle and possibly other Protein 4.1 superfamily members. These studies provide insights into a range of in vivo functions for coracle in developing embryos and adults.
Description
This is the published version, also available here: http://dx.doi.org/10.1091/mbc.9.12.3505.
Date
1998-09-15
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Cell Biology
Collections
Files
Loading...
WardR_MBC_9(12)3505.pdf
Adobe PDF, 1.17 MB
Research Projects
Organizational Units
Journal Issue
Keywords
Citation
Lamb, Rebecca S., Ward, Robert E., Schwizer, Liang., Fehon, Richard G. "Drosophila coracle, a Member of the Protein 4.1 Superfamily, has Essential Structural Functions in the Septate Junctions and Developmental Functions in Embryonic and Adult Epithelial Cells." Mol. Biol. Cell December 1, 1998 vol. 9 no. 12 3505-3519. http://dx.doi.org/10.1091/mbc.9.12.3505.