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Separation and Detection of Peroxynitrite Using Microchip Electrophoresis with Amperometric Detection
Hulvey, Matthew K. Frankenfeld, Celeste Lunte, Susan M.
Hulvey, Matthew K.
Frankenfeld, Celeste
Lunte, Susan M.
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Abstract
Peroxynitrite (ONOO-) is a highly reactive species implicated in the pathology of several cardiovascular and neurodegenerative diseases. It is generated in vivo by the diffusion-limited reaction of nitric oxide (NO•) and superoxide anion (•O2-) and is known to be produced during periods of inflammation. Detection of ONOO- is made difficult by its short half-life under physiological conditions (∼1 s). Here we report a method for the separation and detection of ONOOfrom other electroactive species utilizing a microchip electrophoresis device incorporating an amperometric detection scheme. Microchip electrophoresis permits shorter separation times (∼25 s for ONOO-) and higher temporal resolution than conventional capillary electrophoresis (several minutes). This faster analysis allows ONOO- to be detected before substantial degradation occurs, and the increased temporal resolution
permits more accurate tracking of dynamic changes in chemical systems.
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2010-03-01
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American Chemical Society
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Hulvey, M. K., Frankenfeld, C. N., & Lunte, S. M. (2010). Separation and Detection of Peroxynitrite Using Microchip Electrophoresis with Amperometric Detection. Analytical Chemistry, 82(5), 1608–1611. http://doi.org/10.1021/ac902821v