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Atomic accuracy in predicting and designing non-canonical RNA structure
Das, Rhiju Karanicolas, John Baker, David
Das, Rhiju
Karanicolas, John
Baker, David
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Abstract
We present a Rosetta full-atom framework for predicting and designing the non-canonical motifs that define RNA tertiary structure, called FARFAR (Fragment Assembly of RNA with Full Atom Refinement). For a test set of thirty-two 6-to-20-nucleotide motifs, the method recapitulated 50% of the experimental structures at near-atomic accuracy. Additionally, design calculations recovered the native sequence at the majority of RNA residues engaged in non-canonical interactions, and mutations predicted to stabilize a signal recognition particle domain were experimentally validated.
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2010-02-28
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Nature Publishing Group
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Das, Rhiju, John Karanicolas, and David Baker. “Atomic Accuracy in Predicting and Designing Non-Canonical RNA Structure.” Nature methods 7.4 (2010): 291–294.