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Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro
Sattilaro, Richard F. Dentler, William L., Jr LeCluyse, Edward L.
Sattilaro, Richard F.
Dentler, William L., Jr
LeCluyse, Edward L.
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Abstract
When purified muscle actin was mixed with microtubule-associated proteins (MAPs) prepared from brain microtubules assembled in vitro, actin filaments were organized into discrete bundles, 26 nm in diameter. MAP-2 was the principal protein necessary for the formation of the bundles. Analysis of MAP-actin bundle formation by sedimentation and electrophoresis revealed the bundles to be composed of approximately 20% MAP-2 and 80% actin by weight. Transverse striations were observed to occur at 28-nm intervals along negatively stained MAP-actin bundles, and short projections, approximately 12 nm long and spaced at 28-nm intervals, were resolved by high-resolution metal shadowing. The formation of MAP-actin bundles was inhibited by millimolar concentrations of ATP, AMP-PCP (beta, gamma-methylene-adenosine triphosphate), and pyrophosphate but not by AMP, ADP, or GTP. The addition of ATP to a solution containing MAP-actin bundles resulted in the dissociation of the bundles into individual actin filaments; discrete particles, presumably MAP-2, were periodically attached along the splayed filaments. These results demonstrate that MAPs can bind to actin filaments and can induce the reversible formation of actin filament bundles in vitro.
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This is the publisher's version, also available electronically from http://jcb.rupress.org/content/90/2/467.
Date
1981-08-01
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Rockefeller University Press
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Sattilaro, R. F.; Dentler, William L., Jr; LeCluyse, E. L. (1981). "Microtubule-associated proteins (MAPs) and the organization of actin filaments in vitro." Journal of Cell Biology, 90(2):467-473. http://www.dx.doi.org/10.1083/jcb.90.2.467.