Loading...
Self-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapy
Yazici, Hilal Habib, Gizem Boone, Kyle Urgen, Mustafa Utku, Feride Sermin Tamerler, Candan
Yazici, Hilal
Habib, Gizem
Boone, Kyle
Urgen, Mustafa
Utku, Feride Sermin
Tamerler, Candan
Citations
Altmetric:
Abstract
Bacterial infection is a serious medical problem leading to implant failure. The current antibiotic based therapies rise concerns due to bacterial resistance. The family of antimicrobial peptides (AMP) is one of the promising candidates as local therapy agents due to their broad-spectrum activity. Despite AMPs receive increasing attention to treat infection, their effective delivery to the implantation site has been limited. Here, we developed an engineered dual functional peptide which delivers AMP as a biomolecular therapeutic agent onto calcium phosphate deposited nanotubular titanium surfaces. Dual functionality of the peptide was achieved by combining a hydroxyapatite binding peptide-1 (HABP1) with an AMP using a flexible linker. HABP functionality of the peptide provided a self-coating property onto the nano-topographies that are designed to improve osteointegration capability, while AMP offered an antimicrobial protection onto the implant surface. We successfully deposited calcium phosphate minerals on nanotubular titanium oxide surface using pulse electrochemical deposition (PECD) and characterized the minerals by XRD, FT-IR, FE-SEM. Antimicrobial activity of the engineered peptide was tested against S. mutans (gram- positive) and E. coli (gram-negative) both in solution and on the Ca-P coated nanotubular titanium surface. In solution activity of AMP and dual functional peptide have the same Minimum Inhibitory Concentration (MIC) (32 mg/mL) against E.coli. The peptide also resulted in the reduction of the number of bacteria both for E.coli and S.mutans compare to control groups. Antimicrobial features of dual functional peptides are strongly correlated with their structures suggesting tunability in design through linkers regions. The dual-function peptide offers single-step solution for implant surface functionalization that could be applicable to any implant surface having different topographies.
Description
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Date
2018-09-12
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Research Projects
Organizational Units
Journal Issue
Keywords
Implant infections, Hydroxyapatite-binding peptide, Calcium-phosphate coatings, Nanotubular titanium, Self-assembled peptides, Antimicrobial peptides
Citation
Yazici, H., Habib, G., Boone, K., Urgen, M., Utku, F. S., & Tamerler, C. (2019). Self-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapy. Materials science & engineering. C, Materials for biological applications, 94, 333–343. https://doi.org/10.1016/j.msec.2018.09.030