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Structural and Inhibitor Studies of Norovirus 3C-like Proteases
Takahashi, Daisuke Kim, Yunjeong Lovell, Scott Prakash, Om Chang, Kyeong-Ok
Takahashi, Daisuke
Kim, Yunjeong
Lovell, Scott
Prakash, Om
Chang, Kyeong-Ok
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Abstract
Noroviruses have a single-stranded, positive sense 7–8 kb RNA genome, which encodes a polyprotein precursor processed by a virus-encoded 3C-like cysteine protease (3CLpro) to generate mature non-structural proteins. Because processing of the polyprotein is essential for virus replication, norovirus 3CLpro has been targeted for the discovery of anti-norovirus small molecule therapeutics. Thus, we performed functional, structural and inhibition studies of norovirus 3CLpro with fluorescence resonance energy transfer (FRET) assay, X-ray crystallography, and NMR spectroscopy with a synthetic protease inhibitor. Three 3CLpro from Norwalk virus (NV, genogroup I), MD145 (genogroup II) and murine norovirus-1 (MNV-1, genogroup V) were optimized for a FRET assay, and compared for the inhibitory activities of a synthetic protease inhibitor (GC376). The apo 3D structures of NV 3CLpro determined with X-ray crystallography and NMR spectroscopy were further analyzed. In addition, the binding mode of NV 3CLpro-GC376 was compared with X-ray crystallography and NMR spectroscopy. The results of this report provide insight into the interaction of NV 3CLpro with substrate/inhibitor for better understanding of the enzyme and antiviral drug development.
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2013-09-17
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Elsevier
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Takahashi, Daisuke et al. “Structural and Inhibitor Studies of Norovirus 3C-like Proteases.” Virus research 178.2 (2013): 10.1016/j.virusres.2013.09.008.