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Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases
Lopez Quezada, Landys Smith, Robert Lupoli, Tania J. Edoo, Zainab Li, Xiaojun Gold, Ben Roberts, Julia Ling, Yan Park, Sae Woong Nguyen, Quyen
Lopez Quezada, Landys
Smith, Robert
Lupoli, Tania J.
Edoo, Zainab
Li, Xiaojun
Gold, Ben
Roberts, Julia
Ling, Yan
Park, Sae Woong
Nguyen, Quyen
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Abstract
As β-lactams are reconsidered for the treatment of tuberculosis (TB), their targets are assumed to be peptidoglycan transpeptidases, as verified by adduct formation and kinetic inhibition of Mycobacterium tuberculosis (Mtb) transpeptidases by carbapenems active against replicating Mtb. Here, we investigated the targets of recently described cephalosporins that are selectively active against non-replicating (NR) Mtb. NR-active cephalosporins failed to inhibit recombinant Mtb transpeptidases. Accordingly, we used alkyne analogs of NR-active cephalosporins to pull down potential targets through unbiased activity-based protein profiling and identified over 30 protein binders. None was a transpeptidase. Several of the target candidates are plausibly related to Mtb’s survival in an NR state. However, biochemical tests and studies of loss of function mutants did not identify a unique target that accounts for the bactericidal activity of these beta-lactams against NR Mtb. Instead, NR-active cephalosporins appear to kill Mtb by collective action on multiple targets. These results highlight the ability of these β-lactams to target diverse classes of proteins.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Date
2020-06-23
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Frontiers Media
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Keywords
M. tuberculosis, Cephalosporin, Non-replicating, β-lactams, ABPP, Click chemistry
Citation
Lopez Quezada, L., Smith, R., Lupoli, T. J., Edoo, Z., Li, X., Gold, B., Roberts, J., Ling, Y., Park, S. W., Nguyen, Q., Schoenen, F. J., Li, K., Hugonnet, J. E., Arthur, M., Sacchettini, J. C., Nathan, C., & Aubé, J. (2020). Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases. Frontiers in microbiology, 11, 1248. https://doi.org/10.3389/fmicb.2020.01248