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Role of Glycosylation of IL-1ra on its Binding to IL-1R1
Hutchison, Kevin Michael
Hutchison, Kevin Michael
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Abstract
Interleukin 1 (IL-1) is an inflammatory cytokine that helps the immune system fight disease. The inflammatory action of IL-1 is regulated by the naturally occurring interleukin 1 receptor antagonist (IL-1ra). IL-1ra contains one N-linked glycosylation site and is expressed in humans in both glycosylated and non-glycosylated forms. The one current IL-1ra drug available (Kineret®) is expressed in Escherichia coli as a non-glycosylated form. Because of this, most studies of IL-1ra have been done with the non-glycosylated form and the effects of glycosylation have not been studied. This research attempts to study the effects of the glycosylation of IL-1ra with its binding to the interleukin 1 receptor type 1 (IL-1R1). Both IL-1R1 and IL-1ra were expressed in a glycosylation deficient strain of Pichia pastoris. In-vitro binding experiments between the two proteins were studied using both biolayer interferometry and size exclusion chromatography. Due to the long dissociation rate between IL-1ra and IL-1R1, biolayer interferometry was an unfeasible method. Size exclusion chromatography, however, proved to be a promising tool to study binding. Initial experiments with non-glycosylated IL-1ra showed a potential KD of 1.7 nM. Future studies need to be done using the glycosylated form of IL-1ra to determine any differences in binding between the glycosylated form and the non-glycosylated form.
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Date
2015-12-31
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University of Kansas
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Pharmaceutical sciences