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Acute B-Cell Inhibition by Soluble Antigen Arrays Is Valency-Dependent and Predicts Immunomodulation in Splenocytes
Griffin, J. Daniel Leon, Martin A. Salash, Jean R. Shao, Michael Hartwell, Brittany L. Pickens, Chad J. Sestak, Joshua O. Berkland, Cory
Griffin, J. Daniel
Leon, Martin A.
Salash, Jean R.
Shao, Michael
Hartwell, Brittany L.
Pickens, Chad J.
Sestak, Joshua O.
Berkland, Cory
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Abstract
Antigen valency plays a fundamental role in directing the nature of an immune response to be stimulatory or tolerogenic. Soluble Antigen Arrays (SAgAs) are an antigen-specific immunotherapy that combats autoimmunity through the multivalent display of autoantigen. While mechanistic studies have shown SAgAs to induce T and B-cell anergy, the effect of SAgA valency has never been experimentally tested. Here, SAgAs of discrete antigen valencies were synthesized by click chemistry and evaluated for acute B-cell signaling inhibition as well as downstream immunomodulatory effects in splenocytes. Initial studies using the Raji B-cell line demonstrated SAgA valency dictated the extent of calcium flux. Lower valency constructs elicited the largest reductions in B-cell activation. In splenocytes from mice with experimental autoimmune encephalomyelitis, the same valency-dependent effects were evident in the downregulation of the costimulatory marker CD86. The reduction of calcium flux observed in Raji B-cells correlated strongly with downregulation in splenocyte CD86 expression after 72 hours. Here, a thorough analysis of SAgA antigenic valency illustrates that low, but not monovalent, presentation of autoantigen was ideal for eliciting the most potent immunomodulatory effects.
Description
This document is the Accepted Manuscript version of a Published Work that appeared in final form in
Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.biomac.9b00328.
Date
2019-04-17
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American Chemical Society
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Griffin_2019.pdf
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Keywords
Soluble Antigen Array, Antigen-Specific Immunotherapy, Valency, Calcium flux, Anergy, Click Chemistry
Citation
Griffin, J. D., Leon, M. A., Salash, J. R., Shao, M., Hartwell, B. L., Pickens, C. J., Sestak, J. O., & Berkland, C. (2019). Acute B-Cell Inhibition by Soluble Antigen Arrays Is Valency-Dependent and Predicts Immunomodulation in Splenocytes. Biomacromolecules, 20(5), 2115–2122. https://doi.org/10.1021/acs.biomac.9b00328