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Illuminating the diversity of aromantic polyketide synthases in Aspergillus nidulans
Ahuja, Manmeet Chiang, Yi-Ming Chang, Shu-Lin Praseuth, Mike B. Entwistle, Ruth A. Sanchez, James F. Lo, Hsien-Chun Yeh, Hsu-Hua Oakley, Berl R. Wang, Clay C. C.
Ahuja, Manmeet
Chiang, Yi-Ming
Chang, Shu-Lin
Praseuth, Mike B.
Entwistle, Ruth A.
Sanchez, James F.
Lo, Hsien-Chun
Yeh, Hsu-Hua
Oakley, Berl R.
Wang, Clay C. C.
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Abstract
Genome sequencing has revealed that fungi have the ability to synthesize many more natural products (NPs) than are currently known, but methods for obtaining suitable expression of NPs have been inadequate. We have developed a successful strategy that bypasses normal regulatory mechanisms. By efficient gene targeting, we have replaced, en masse, the promoters of non-reducing polyketide synthase (NR-PKS) genes, key genes in NP biosynthetic pathways and other genes necessary for NR-PKS product formation or release. This has allowed us to determine the products of eight NR-PKSs of A. nidulans, including seven novel compounds, as well as the NR-PKS genes required for the synthesis of the toxins, alternariol (8) and cichorine (19).
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This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/ja3016395.
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2012-05-16
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American Chemical Society
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Ahuja, M., Chiang, Y.-M., Chang, S.-L., Praseuth, M. B., Entwistle, R., Sanchez, J. F., … Wang, C. C. C. (2012). Illuminating the diversity of aromatic polyketide synthases in Aspergillus nidulans. Journal of the American Chemical Society, 134(19), 8212–8221. http://doi.org/10.1021/ja3016395