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Microdialysis Sampling Coupled to Microchip Electrophoresis with Integrated Amperometric Detection on an All Glass Substrate
Scott, David E. Grigsby, Ryan Lunte, Susan M.
Scott, David E.
Grigsby, Ryan
Lunte, Susan M.
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Abstract
The development of an all-glass separation-based sensor using microdialysis coupled to microchip electrophoresis with amperometric detection is described. The system includes a flow-gated interface to inject discrete sample plugs from the microdialysis perfusate into the microchip electrophoresis system. Electrochemical detection was accomplished with a platinum electrode in an in-channel configuration using a wireless electrically isolated potentiostat. To facilitate bonding around the in-channel electrode, a fabrication process was employed that produced a working and a reference electrode flush with the glass surface. Both normal and reversed polarity separations were performed with this sensor. The system was evaluated in vitro for the continuous monitoring of the production of hydrogen peroxide from the reaction of glucose oxidase with glucose. Microdialysis experiments were performed using a BASi loop probe with an overall lag time of approximately five minutes and a rise time of less than 60 seconds.
Description
This is the peer reviewed version of the following article: Scott, D. E., Grigsby, R., & Lunte, S. M. (2013). Microdialysis Sampling Coupled to Microchip Electrophoresis with Integrated Amperometric Detection on an All Glass Substrate. Chemphyschem : A European Journal of Chemical Physics and Physical Chemistry, 14(10), 2288–2294. http://doi.org/10.1002/cphc.201300449, which has been published in final form at doi.org/10.1002/cphc.201300449. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Date
2013-07-22
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Wiley
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Keywords
Glass microchip, Electrochemical detection, Microchip electrophoresis, Microdialysis, Microfluidics
Citation
Scott, D. E., Grigsby, R., & Lunte, S. M. (2013). Microdialysis Sampling Coupled to Microchip Electrophoresis with Integrated Amperometric Detection on an All Glass Substrate. Chemphyschem : A European Journal of Chemical Physics and Physical Chemistry, 14(10), 2288–2294. http://doi.org/10.1002/cphc.201300449