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Evaluation of the role of P-glycoprotein in ivermectin uptake by primary cultures of bovine brain microvessel endothelial cells

Rose, Jayna M.
Peckham, Sara L.
Scism, Jamie L.
Audus, Kenneth L.
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Abstract
The P-glycoprotein efflux system located on the apical membrane of brain capillary endothelial cells functions as part of the blood-brain barrier. In this study, primary cultures of bovine brain microvessel endothelial cells (BMECs) were investigated for the presence of a P-glycoprotein system and its contribution in regulating ivermectin distribution across the blood-brain barrier. Results of rhodamine 123 uptake studies with cyclosporin A and verapamil as substrates indicated that a functional efflux system was present on BMECs. Immunoblot analysis with the C219 monoclonal antibody to the product of the multidrug resistant member 1(MDR1) gene also confirmed the expression of MDR1 in the BMECs. Unbound ivermectin was shown to significantly increase the uptake of rhodamine 123 in BMECs, however, the drug only modestly enhanced the transcellular passage of rhodamine. The results of these studies affirmed that unbound ivermectin is an inhibitor of the MDR1 efflux system in BMECs.
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Date
1998
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Publisher
Springer Verlag
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Keywords
Brain microvessel endothelial cells, Blood-brain barrier, P-glycoprotein, Rhodamine 123, ivermectin
Citation
Rose, J.M., Peckham, S.L., Scism, J.L., and Audus, K.L. (1998) Evaluation of the role of P-glycoprotein in ivermectin uptake by primary cultures of bovine brain microvessel endothelial cells. Neurochem. Res. 23, 203-209. PMID: 9475515 http://dx.doi.org/10.1023/A:1022485026198
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