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Chlamydia trachomatis protein CT009 is a structural and functional homolog to the key morphogenesis component RodZ and interacts with division septal plane localized MreB
Kemege, Kyle Evan Hickey, John M. Barta, Michael L. Wickstrum, Jason Balwalli, Namita Ashwin Lovell, Scott Battaile, Kevin P. Hefty, P. Scott
Kemege, Kyle Evan
Hickey, John M.
Barta, Michael L.
Wickstrum, Jason
Balwalli, Namita Ashwin
Lovell, Scott
Battaile, Kevin P.
Hefty, P. Scott
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Abstract
Cell division in Chlamydiae is poorly understood as apparent homologs to most conserved bacterial cell division proteins are lacking and presence of elongation (rod shape) associated proteins indicate non-canonical mechanisms may be employed. The rod-shape determining protein MreB has been proposed as playing a unique role in chlamydial cell division. In other organisms, MreB is part of an elongation complex that requires RodZ for proper function. A recent study reported that the protein encoded by ORF CT009 interacts with MreB despite low sequence similarity to RodZ. The studies herein expand on those observations through protein structure, mutagenesis, and cellular localization analyses. Structural analysis indicated that CT009 shares high level of structural similarity to RodZ, revealing the conserved orientation of two residues critical for MreB interaction. Substitutions eliminated MreB protein interaction and partial complementation provided by CT009 in RodZ deficient E. coli. Cellular localization analysis of CT009 showed uniform membrane staining in Chlamydia. This was in contrast to the localization of MreB, which was restricted to predicted septal planes. MreB localization to septal planes provides direct experimental observation for the role of MreB in cell division and supports the hypothesis that it serves as a functional replacement for FtsZ in Chlamydia.
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This is the peer reviewed version of the following article: Kemege, K. E., Hickey, J. M., Barta, M. L., Wickstrum, J., Balwalli, N., Lovell, S., Battaile, K. P. and Hefty, P. S. (2015), Chlamydia trachomatis protein CT009 is a structural and functional homolog to the key morphogenesis component RodZ and interacts with division septal plane localized MreB. Molecular Microbiology, 95: 365–382. doi:10.1111/mmi.12855, which has been published in final form at http://doi.org/10.1111/mmi.12855. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Date
2015-02
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Wiley
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Keywords
Chlamydia, RodZ, Morphogenesis, Structure
Citation
Kemege, K. E., Hickey, J. M., Barta, M. L., Wickstrum, J., Balwalli, N., Lovell, S., Battaile, K. P. and Hefty, P. S. (2015), Chlamydia trachomatis protein CT009 is a structural and functional homolog to the key morphogenesis component RodZ and interacts with division septal plane localized MreB. Molecular Microbiology, 95: 365–382. doi:10.1111/mmi.12855