Loading...
The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS)
Gerdts, Cory. J Elliot, Mark Lovell, Scott Mixon, Mark B. Napuli, Alberto J. Staker, Bart L. Nollert, Peter Stewart, Lance
Gerdts, Cory. J
Elliot, Mark
Lovell, Scott
Mixon, Mark B.
Napuli, Alberto J.
Staker, Bart L.
Nollert, Peter
Stewart, Lance
Citations
Altmetric:
Abstract
The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, ~10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comÂprehensive `hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.
Description
This is the published version. Copyright International Union of Crystallography
Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
International Union of Crystallography
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
Protein crystallization, Microcapillary protein crystallization system
Citation
Gerdts, Cory J., Mark Elliott, Scott Lovell, Mark B. Mixon, Alberto J. Napuli, Bart L. Staker, Peter Nollert, and Lance Stewart. "The Plug-based Nanovolume Microcapillary Protein Crystallization System (MPCS)." Acta Crystallogr D Biol Cryst Acta Cryst D Acta Crystallogr Sect D Biol Crystallogr Acta Crystallogr Sect D Acta Crystallogr D Biol Crystallogr Acta Crystallographica Section D Biological Crystallography Acta Cryst Sect D Acta Crystallogr D 64.11 (2008): 1116-122. http//dx.doi.org/10.1107/S0907444908028060