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Hyaluronic acid graft polymers displaying peptide antigen madulate dendritic cell response in vitro
Chittasupho, Chuda Sestak, Joshua Shannon, Laura Siahaan, Teruna J. Vines, Charlotte M. Berkland, Cory J.
Chittasupho, Chuda
Sestak, Joshua
Shannon, Laura
Siahaan, Teruna J.
Vines, Charlotte M.
Berkland, Cory J.
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Abstract
A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA, significantly suppressed T cell and DC conjugate formation, T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity.
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Date
2014-01-06
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American Chemical Society
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Keywords
Peptides, Hyaluronic acid, Targeted delivery, Dendritic cells, T cells
Citation
Chittasupho, C., Sestak, J., Shannon, L., Siahaan, T. J., Vines, C. M., & Berkland, C. (2014). Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro. Molecular Pharmaceutics, 11(1), 367–373. http://doi.org/10.1021/mp4003909