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Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
Asad, Nadeem Smith, Emily Shakya, Sudeep Stegman, Sutton Timmons, Lisa
Asad, Nadeem
Smith, Emily
Shakya, Sudeep
Stegman, Sutton
Timmons, Lisa
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Abstract
We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.
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2023-03-06
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MDPI
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Asad_2023.pdf
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Keywords
Electrophoresis, Agarose, Ethidium bromide, Sustainability
Citation
Asad, N.; Smith, E.; Shakya, S.; Stegman, S.; Timmons, L. Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids. Methods Protoc. 2023, 6, 25. https://doi.org/10.3390/mps6020025