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Crystallization and Preliminary X-Ray Analysis of Human Muscle Creatine Kinase

Tang, Liang
; 
Zhou, Hai-Meng
; 
Lin, Zheng-jiong
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Abstract
Creatine kinase is a key enzyme in the energy homeostasis of cells and tissues with high and fluctuating energy demands. Human muscle MM creatine kinase is a dimeric protein with a molecular weight of \sim43 kDa for each subunit. It has been crystallized by the hanging-drop vapor-diffusion method using 2-methyl-2,4-pentanediol as precipitant. The crystals belong to the enantiomorphous space group P6_222 or P6_422 with cell parameters of a=b=89.11 and c=403.97 Å. The asymmetric unit of the crystal contains two subunits. A data set at 3.3 Å resolution has been collected using synchrotron radiation.
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This is the publisher's version, also available electronically from "http://scripts.iucr.org".
Date
1999-03-01
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Publisher
International Union of Crystallography
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Molecular Biosciences Scholarly Works
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TangLiang_AC_1999(55)669.pdf
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Keywords
creatine kinase, synchrotron radiation
Citation
Tang, L., Zhou, H., & Lin, Z. (1999). Crystallization and preliminary X-ray analysis of human muscle creatine kinase. Acta Crystallographica Section D Biological Crystallography, 55(3), 669-670. http://www.dx.doi.org/10.1107/S0907444998011044
URI
https://hdl.handle.net/1808/17592
DOI
10.1107/S0907444998011044
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